Formulation of Dermal Tissue Matrix Bioink by a Facile Decellularization Method and Process Optimization for 3D Bioprinting toward Translation Research

Decellularized extracellular matrices (ECMs) are being extensively used for tissue engineering purposes and detergents are predominantly used for this. A facile detergent‐free decellularization method is developed for dermal matrix and compared it with the most used detergent‐based decellularization...

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Bibliographic Details
Published in:Macromolecular bioscience 2022-08, Vol.22 (8), p.e2200109-n/a
Main Authors: Bera, Ashis Kumar, Sriya, Yeleswarapu, Pati, Falguni
Format: Article
Language:English
Subjects:
Biocompatibility
bioprinting process optimization
Collagen
Deoxyribonucleic acid
dermal tissues
Detergents
detergent‐free decellularization
DNA
Extracellular matrix
Flow rates
Flow velocity
Glycosaminoglycans
hypertonic/hypotonic NaCl solutions
isotropic cellular alignment
Optimization
Shear thinning (liquids)
Skin
Sodium chloride
Three dimensional printing
Tissue engineering
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Summary:Decellularized extracellular matrices (ECMs) are being extensively used for tissue engineering purposes and detergents are predominantly used for this. A facile detergent‐free decellularization method is developed for dermal matrix and compared it with the most used detergent‐based decellularization methods. An optimized, single‐step, cost‐effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions‐based method is employed to decellularize goat skin that resulted in much higher yield than other methods. The ECM composition, mechanical property, and cytocompatibility are evaluated and compared with other decellularization methods. Furthermore, this H/H‐treated decellularized dermal ECM (ddECM) exhibits a residual DNA content of
ISSN:1616-5187
1616-5195
DOI:10.1002/mabi.202200109