Frequency of natural antibodies and concordance analysis for anti-TOXOPLASMA IgM tests in Colombian sera of pregnant women

Interpretation of IgM anti-Toxoplasma can be problematic given the phenomena of “natural” IgM. We analyzed 1,503 sera obtained during prenatal care program, and we established natural and false-positive results by doing follow-up. In 101 samples the concordance between enzyme linked immunosorbent as...

Full description

Saved in:
Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2022-08, Vol.103 (4), p.115733-115733, Article 115733
Main Authors: Plazas, María Inés, Salamanca-Marin, Jacqueline, Torres-Morales, Elizabeth, Londoño, Juan Camilo, Celis-Giraldo, Daniel, Gomez-Marin, Jorge Enrique
Format: Article
Language:English
Subjects:
chemiluminescence
diagnosis
ELFA
ELISA
IgM anti-Toxoplasma
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Interpretation of IgM anti-Toxoplasma can be problematic given the phenomena of “natural” IgM. We analyzed 1,503 sera obtained during prenatal care program, and we established natural and false-positive results by doing follow-up. In 101 samples the concordance between enzyme linked immunosorbent assay (ELISA) and two semi-automatized systems: electro-chemiluminescence immunoassay (ELECSYS) and vitek immunodiagnostic assay system (VIDAS) was calculated. In 1,503 serum, 71 (4,7%) had ELISA IgG negative and ELISA IgM positive results and in 44 of these had a second sample 4 weeks later. In second samples, 27 (61,3 %) were IgM and IgG negative (false positive result in the first sample) and 13 (29,5%) were ELISA IgM positive and IgG negative (natural IgM). ELISA assay had a poor concordance with enzyme-linked fluorescent immunoassay as well as ELECSYS tests, contrarily, enzyme-linked fluorescent immunoassay and ELECSYS had optimal concordance, with 100 of 101 sera obtaining the same result by both tests. We recommended to use automatized assays to measure IgM anti-Toxoplasma.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2022.115733