Significance of Cyclooxgenase-2 gene polymorphism and related miRNAs in pulmonary arterial hypertension

[Display omitted] Pulmonary arterial hypertension (PAH) is a rare disease with a poor prognosis. The suppression of cyclooxygenase-2 (COX-2) expression has been known to impair vascular function in endothelial cells; however, the epigenetic factors that cause this are largely obscure. Our aim in thi...

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Published in:Clinical biochemistry 2022-09, Vol.107, p.33-39
Main Authors: Durmus, Sinem, Atahan, Ersan, Avci Kilickiran, Burcak, Onal, Burak, Cakatay, Ufuk, Gelisgen, Remise, Uzun, Hafize
Format: Article
Language:English
Subjects:
Cyclooxgenase-2
miRNA
Polymorphism
Pulmonary arterial hypertension
SNP
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Summary:[Display omitted] Pulmonary arterial hypertension (PAH) is a rare disease with a poor prognosis. The suppression of cyclooxygenase-2 (COX-2) expression has been known to impair vascular function in endothelial cells; however, the epigenetic factors that cause this are largely obscure. Our aim in this study was to examine the polymorphisms in the gene for COX-2 (PTGS2) and related miRNAs regulating its level in a single-center cohort of patients with PAH. In this study, three SNPs and miRNAs (rs5275, rs689470, rs20417, miR-26b-5p, miR-146a-5p, and miR-101-5p) in the PTGS2 were screened in PAH and controls by qPCR. In addition, the COX-2 level was determined by immunoassay to examine the effects of epigenetic factors on its expression levels. The non-dominant genotypes of rs20417 and rs5275 were found to be related to PAH (OR = 8.56, 95% CI = 3.39–21.63, p 
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2022.06.001