Inhibitory activity of soybean (Glycine max L. Merr.) Cell Culture Extract on tyrosinase activity and melanin formation in alpha-melanocyte stimulating Hormone-Induced B16-F10 melanoma cells

• Published in: Molecular biology reports 2022-08, Vol.49 (8), p.7827-7836
• Main Authors: Bodurlar, Yildiz, Caliskan, Mahmut
• Format: Article
• Language: English
• Subjects: a-Melanocyte-stimulating hormone; alpha-MSH - genetics; alpha-MSH - metabolism; alpha-MSH - pharmacology; Animal Anatomy; Animal Biochemistry; Animals; Biomedical and Life Sciences; Cell culture; Cell Culture Techniques; Cell Extracts; Cell Line, Tumor; Cell viability; Contact dermatitis; Glycine max; Glycine max - metabolism; Histology; Hyperpigmentation; Irritation; Life Sciences; Melanin; Melanins - metabolism; Melanocyte-stimulating hormone; Melanoma; Melanoma, Experimental - drug therapy; Melanoma, Experimental - metabolism; Mice; Microphthalmia-associated transcription factor; Microphthalmia-Associated Transcription Factor - genetics; Microphthalmia-Associated Transcription Factor - metabolism; Monophenol Monooxygenase; Morphology; Original Article; Plant Extracts - pharmacology; Proteins; Side effects
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-022-07608-6

Background Hyperpigmentation, which causes excessive melanin synthesis and accumulation, is an important issue in the cosmetic industry. Since compounds developed against hyperpigmentation often come with side effects such as skin irritation and contact dermatitis, new studies focus on the use of natural agents that have no side effects. Methods and Results In this study, it was found that the effects of soybean cell culture extract (SCE) on alpha-melanocyte-stimulating hormone (α-MSH) induced melanogenesis in B16-F10 murine melanoma cells. The cells were incubated with SCE for 48 h after treatment with α‑MSH for 24 h to analysis the melanin content, cellular tyrosinase activity, and gene and protein expression. SCE at 1 mg/mL decreased melanin content and tyrosinase activity by 34% and 24%, respectively, compared to the α-MSH-treated group, which did not decrease cell viability. In addition, SCE (1 mg/mL) downregulated the expression of tyrosinase (TYR), tyrosinase-related protein (TRP)-1, tyrosinase-related protein (TRP)-2, and microphthalmia-associated transcription factor (MITF) genes 1.5-, 1.5-, 2-, and 2-fold, respectively. Furthermore, SCE inhibited the expression of TYR, TRP1, and TRP2 by decreasing the expression of MITF, as shown in a western blot assay. Conclusions This study suggests that SCE reveals dose-dependent inhibition of melanin synthesis through the suppression of tyrosinase activity as well as molecular levels of TYR, TRP1, TRP2, and MITF in B16-F10 murine melanoma cells. Therefore, SCE has the potential to be an effective and natural skin-whitening agent for application in the cosmetic industry.