Nrf2 pathway in vegetable oil-induced inflammation of large yellow croaker (Larimichthys crocea)

This study was conducted to investigate the effects and regulation of dietary vegetable oil (VO, enriched with α-linolenic acid [ALA] and linoleic acid [LNA]) on the nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor-κB (NF-κB) pathways in large yellow croaker. In vivo study showe...

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Published in:Fish & shellfish immunology 2022-08, Vol.127, p.778-787
Main Authors: Tan, Peng, Ding, Yi, Li, Xueshan, Dong, Xiaojing, Mai, Kangsen, Ai, Qinghui
Format: Article
Language:English
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Fish oil
Larimichthys crocea
Nuclear factor erythroid 2-related factor 2
Nuclear factor-κB
Vegetable oil
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creator Tan, Peng
Ding, Yi
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Ai, Qinghui
description This study was conducted to investigate the effects and regulation of dietary vegetable oil (VO, enriched with α-linolenic acid [ALA] and linoleic acid [LNA]) on the nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor-κB (NF-κB) pathways in large yellow croaker. In vivo study showed that the VO diet significantly decreased the activity of antioxidant enzymes and antioxidant enzyme-related mRNA expression in the liver tissue, in comparison with the fish oil (FO) diet (P 
doi_str_mv 10.1016/j.fsi.2022.05.046
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In vivo study showed that the VO diet significantly decreased the activity of antioxidant enzymes and antioxidant enzyme-related mRNA expression in the liver tissue, in comparison with the fish oil (FO) diet (P &lt; 0.05). The suppression of antioxidant capacity might be due to the decrease of nuclear Nrf2 protein translocation, Nrf2 binding to antioxidant response element (ARE) sequences, and subsequently, antioxidant genes transcription as electrophoretic mobility shift assay (EMSA) and luciferase assay showed. VO-derivated ALA and LNA exerted a lower antioxidant capacity than FO-derivated DHA and EPA, characterized by significantly lower nucleus Nfr2 protein expression but significantly higher ROS production values in primary hepatocytes (P &lt; 0.05). The pro-inflammatory genes (tumor necrosis factor α [TNFα] and interleukin 1β [IL1β]) expression was significantly higher in the liver tissue of fish fed the VO diet which might be due to the activation of the NF-κB pathway (P &lt; 0.05). Knockdown of the Nrf2 gene negatively affected the anti-inflammatory effect of fatty acids by increasing the expression of TNFα and the IL1β gene and nuclear p65 protein (P &lt; 0.05). In general, the results indicated that dietary vegetable oil decreased antioxidant capacity but induced inflammatory responses through the Nrf2/NF-κB pathway. [Display omitted] •VO decreased nuclear Nrf2 protein translocation and binding to ARE sequences.•DHA and EPA exerted higher antioxidant capacity than ALA and LNA in primary hepatocytes.•VO increased pro-inflammatory genes expression through the NF-κB pathway.•Knockdown of the Nrf2 gene knockdown downregulated fatty acids anti-inflammatory effect.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2022.05.046</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Fish oil ; Larimichthys crocea ; Nuclear factor erythroid 2-related factor 2 ; Nuclear factor-κB ; Vegetable oil</subject><ispartof>Fish &amp; shellfish immunology, 2022-08, Vol.127, p.778-787</ispartof><rights>2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c260t-6fb6e8af85024f5d0a3a0c1cbfb5deb22d086f25fb0115c07c4a19031f94f4fb3</citedby><cites>FETCH-LOGICAL-c260t-6fb6e8af85024f5d0a3a0c1cbfb5deb22d086f25fb0115c07c4a19031f94f4fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Tan, Peng</creatorcontrib><creatorcontrib>Ding, Yi</creatorcontrib><creatorcontrib>Li, Xueshan</creatorcontrib><creatorcontrib>Dong, Xiaojing</creatorcontrib><creatorcontrib>Mai, Kangsen</creatorcontrib><creatorcontrib>Ai, Qinghui</creatorcontrib><title>Nrf2 pathway in vegetable oil-induced inflammation of large yellow croaker (Larimichthys crocea)</title><title>Fish &amp; shellfish immunology</title><description>This study was conducted to investigate the effects and regulation of dietary vegetable oil (VO, enriched with α-linolenic acid [ALA] and linoleic acid [LNA]) on the nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor-κB (NF-κB) pathways in large yellow croaker. 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The pro-inflammatory genes (tumor necrosis factor α [TNFα] and interleukin 1β [IL1β]) expression was significantly higher in the liver tissue of fish fed the VO diet which might be due to the activation of the NF-κB pathway (P &lt; 0.05). Knockdown of the Nrf2 gene negatively affected the anti-inflammatory effect of fatty acids by increasing the expression of TNFα and the IL1β gene and nuclear p65 protein (P &lt; 0.05). In general, the results indicated that dietary vegetable oil decreased antioxidant capacity but induced inflammatory responses through the Nrf2/NF-κB pathway. 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In vivo study showed that the VO diet significantly decreased the activity of antioxidant enzymes and antioxidant enzyme-related mRNA expression in the liver tissue, in comparison with the fish oil (FO) diet (P &lt; 0.05). The suppression of antioxidant capacity might be due to the decrease of nuclear Nrf2 protein translocation, Nrf2 binding to antioxidant response element (ARE) sequences, and subsequently, antioxidant genes transcription as electrophoretic mobility shift assay (EMSA) and luciferase assay showed. VO-derivated ALA and LNA exerted a lower antioxidant capacity than FO-derivated DHA and EPA, characterized by significantly lower nucleus Nfr2 protein expression but significantly higher ROS production values in primary hepatocytes (P &lt; 0.05). The pro-inflammatory genes (tumor necrosis factor α [TNFα] and interleukin 1β [IL1β]) expression was significantly higher in the liver tissue of fish fed the VO diet which might be due to the activation of the NF-κB pathway (P &lt; 0.05). Knockdown of the Nrf2 gene negatively affected the anti-inflammatory effect of fatty acids by increasing the expression of TNFα and the IL1β gene and nuclear p65 protein (P &lt; 0.05). In general, the results indicated that dietary vegetable oil decreased antioxidant capacity but induced inflammatory responses through the Nrf2/NF-κB pathway. 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subjects Fish oil
Larimichthys crocea
Nuclear factor erythroid 2-related factor 2
Nuclear factor-κB
Vegetable oil
title Nrf2 pathway in vegetable oil-induced inflammation of large yellow croaker (Larimichthys crocea)
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