Calcium controlled NFATc1 activation enhances suppressive capacity of regulatory T cells isolated from generalized vitiligo patients

NFATs and FOXP3 are linked with impaired regulatory T‐cells (Tregs) in generalized vitiligo (GV). To elucidate calcium mediated NFATc1 signalling pathway and its effect on Treg suppressive capacity in GV. Calcium levels, calcineurin, NFATc1 and GSK‐3β activity and cell proliferation were assessed in...

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Bibliographic Details
Published in:Immunology 2022-11, Vol.167 (3), p.314-327
Main Authors: Giri, Prashant S., Bharti, Ankit H., Begum, Rasheedunnisa, Dwivedi, Mitesh
Format: Article
Language:English
Subjects:
Assaying
Calcineurin
Calcineurin phosphatase
Calcium
Calcium (blood)
Calcium (intracellular)
Calcium channels
Calcium homeostasis
Calcium influx
Calcium signalling
calmodulin
CD4 antigen
CD8 antigen
Cell growth
Cell proliferation
Cytokines
DYRK1A
Enzyme-linked immunosorbent assay
FOXP3
Foxp3 protein
GSK‐3β
Homeostasis
immune suppression
Immunoregulation
Interferon
Intracellular
Lymphocytes
Lymphocytes T
NFATc1
ORAI1
Orai1 protein
Patients
regulatory T‐cells (Tregs)
Signal transduction
Signaling
Skin diseases
Vitiligo
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Summary:NFATs and FOXP3 are linked with impaired regulatory T‐cells (Tregs) in generalized vitiligo (GV). To elucidate calcium mediated NFATc1 signalling pathway and its effect on Treg suppressive capacity in GV. Calcium levels, calcineurin, NFATc1 and GSK‐3β activity and cell proliferation were assessed in 52 GV patients and 50 controls by calcium assay kit, calcineurin phosphatase assay kit, TransAM NFATc1 kit, GSK‐3β ELISA and BrdU cell proliferation assay. Transcripts (CNB, CAM, GSK3B, DYRK1A and calcium channel genes) and protein (IFN‐γ, IL‐10 and TGF‐β) expressions were assessed by qPCR and ELISA, respectively. Reduced plasma and intracellular Tregs calcium levels and ORAI1 transcripts suggested altered calcium homeostasis in GV Tregs (p = 0.00387, p = 0.0048, p 
ISSN:0019-2805
1365-2567
DOI:10.1111/imm.13538