Proteomic analysis of chemically transformed NIH-3T3 cells reveals novel mechanisms of action of amaranth lunasin-like peptide

• Published in: Food research international 2022-07, Vol.157, p.111374-111374, Article 111374
• Main Authors: Mazorra-Carrillo, Jorge L., De León-Rodríguez, Antonio, Huerta-Ocampo, José A., Velarde-Salcedo, Aída J., González de Mejía, Elvira, Barba de la Rosa, Ana P.
• Format: Article
• Language: English
• Subjects: Amaranth seeds; Bioactive peptides; Chemical transformation; Gel-based proteomics; NIH-3T3 cells
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2022.111374

[Display omitted] •2-DE gel-based proteomics was a powerful technique to analyze AhLun molecular action.•Down-accumulation of hnRNPQ, a cancer biomarker, was regulated in AhLun treatments.•Gal3, associated with tumour progression, was regulated in AhLun treatments.•Different isoforms of Prelamin A/C were detected, indicating the importance of posttranslational modifications. Food-derived biopeptides can interact with genes and proteins to preserve health and prevent the development of diseases. Lunasin is a soybean cancer-preventive peptide that has been well characterized; however, few studies have been carried out to characterize the function of amaranth lunasin-like peptide (AhLun). The aim of this work was to analyze the proteomic profile changes in NIH-3T3 cells when they are chemically transformed with the carcinogen 3-methylcholanthrene (3MC) in the absence or presence of AhLun. The addition of AhLun into the culture medium did not affect the cell morphology; however, as a chemopreventive agent, it significantly reduced anisokaryosis formation when cells were treated with 3MC. Changes in protein accumulation in NIH-3T3 cells were evaluated by gel-based proteomics analysis. Differentially accumulated protein spots that exhibited at least a twofold change in spot intensity (p