Effects of differently incubated cupric oxide nanoparticles on the granulosa cells of caprine ovary in vitro

• Published in: Environmental science and pollution research international 2022-12, Vol.29 (56), p.84243-84255
• Main Authors: Kumar, Chetan, Sharma, Rajnesh Kumar
• Format: Article
• Language: English
• Subjects: Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Copper; Copper oxides; Cytotoxicity; Diffusion rate; Drug delivery; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Granulosa cells; Melt temperature; Metal oxides; Nanoparticles; Nanotechnology; Precipitates; Reduction (metal working); Research Article; Sodium hydroxide; Surface chemistry; Temperature; Temperature effects; Toxicity; Toxicology; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-022-21691-z

In the nanoscience metal and metal oxide, nanoparticles have a prominent place because of their vast applications. Recent finding shows that in addition to size, there are other critical factors governing the biological response of nanoparticles. These factors include surface chemistry and shape that influences solubility, rate of diffusion, drug delivery, melting temperature, and colour of the nanoparticles. It is thus the present study that was aimed to investigate the effect of temperature on the shape and size of nanoparticles and related cytotoxicity of these particles on ovarian granulosa cells. Cupric oxide nanoparticles (CuONPs) were synthesized using a simple, efficient, and reproducible precipitation method involving the reduction of Cu metal salt with sodium hydroxide and then incubation of the precipitates at 70 °C for 5 h. Subsequently, this prepared sample was divided into 3 subsamples and incubated at 3 different temperatures, i.e. 70 °C, 150 °C, and 350 °C for 5 h to study the effect of temperature on the particles. The products were characterized by XRD, FTIR, HRTEM, and FESEM. Characterization of the particles revealed that all particles were monoclinic crystalline in nature and had a size range from 9 to 60 nm. Particles were of different shapes: spherical, needle, and capsule. The toxicity of each particle was determined on granulosa cells by exposing cells for 24 h at 2 different doses. Toxicological results showed the size and shape-related toxicity of nanoparticles where spherical shapes were significantly more toxic than capsule-shaped particles.