Protein engineering for electrochemical biosensors

The development of electrochemical biosensors has gained tremendous attention. Protein engineering has been applied for enhancing properties of native redox enzymes, such as selectivity, sensitivity, and stability required for applicable biosensors. This review highlights recent advances of protein...

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Bibliographic Details
Published in:Current opinion in biotechnology 2022-08, Vol.76, p.102751-102751, Article 102751
Main Authors: Zhu, Zhiguang, Song, Haiyan, Wang, Yuanming, Zhang, Yi-Heng PJ
Format: Article
Language:English
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Summary:The development of electrochemical biosensors has gained tremendous attention. Protein engineering has been applied for enhancing properties of native redox enzymes, such as selectivity, sensitivity, and stability required for applicable biosensors. This review highlights recent advances of protein engineering to improve enzymatic catalysis of biosensors, facilitate electron transfer and enzyme immobilization, and construct allosteric protein biosensors. The pros and cons of different protein engineering strategies are briefly discussed, and perspectives are further provided. [Display omitted] •Protein engineering has been widely used to improve biosensor performance.•Enzymatic catalysis and electron transfer both play essential roles.•Oriented enzyme immobilization at enzyme–electrode interface is highly desirable.•Direct electron transfer-based 3rd generation biosensor is the future direction.
ISSN:0958-1669
1879-0429
DOI:10.1016/j.copbio.2022.102751