Aerobic exercise improves adipogenesis in diet-induced obese mice via the LncSRA/p38/JNK/PPARγ pathway

• Published in: Nutrition research (New York, N.Y.) N.Y.), 2022-09, Vol.105, p.20-32
• Main Authors: Wu, Baoai, Ding, Jiyuan, Chen, Anping, Song, Yicun, Xu, Cong, Tian, Fujun, Zhao, Jinfeng
• Format: Article
• Language: English
• Subjects: Aerobic exercise; LncRNA-SRA; obesity; p38/JNK; PPARγ
• ISSN: 0271-5317; 1879-0739
• DOI: 10.1016/j.nutres.2022.04.004

Adipogenesis is one of the triggers of obesity, which is a risk factor for various metabolic diseases. LncRNA-SRA is closely related to adipogenesis and p38/JNK mitogen-activated protein kinase (p38/JNK MAPK) mediates lipid production by regulating PPARγ. Aerobic exercise can be efficient in improving adiposity and losing weight. Hence, we hypothesize that aerobic exercise ameliorates obesity by affecting the SRA/p38/JNK/PPARγ pathway and downstream target genes. The broad approaches used to test hypotheses are as follows. Spectrophotometer detected C57BL/6J mice blood lipid level; HE stained fat tissue to check the grade of epididymis fat; QPCR and Western blot detected mRNA expression and protein levels. Injected LncRNA-SRA virus vector to over-express SRA. After 8 weeks of aerobic exercise intervention, obese mice showed significant improvements in body weight, white fat weight, lipid levels, and Lee's index. Aerobic exercise significantly inhibited he expression of SRA, activated the p38/JNK signaling pathway, further inhibited the expression of PPARγ and downstream target genes, and improved obesity. Aerobic exercise intervention improved lipid metabolism in obese mice, and the mechanism may be related to the regulation of the LncSRA/p38/JNK/PPARγ signaling pathway. Aerobic exercise is a globally recognized method for weight loss, and aerobic exercise successfully attenuates obesity by suppressing LncSRA expression caused by a high-fat diet. When LncSRA is suppressed, it activates the p38/JNK signaling pathway and further suppresses PPARγ and its downstream target genes, such as FAS, aP2, PCK, and GK. Hence, aerobic exercise inhibits LncSRA expression and further activates p38/JNK signaling pathway to suppress PPARγ and its targets expression to improve adipogenesis. [Display omitted]