Presence of quorum sensing system, virulence genes, biofilm formation and relationship among them and class 1 integron in carbapenem-resistant clinical Pseudomonas aeruginosa isolates

Carbapenems are the most effective agents for treating clinical P. aeruginosa (PsA) infections. During an infection, a quorum-sensing (QS) system and its regulating virulence genes have a great role. The aim of the study was to detect the presence of a las and rhl QS system and related virulence gen...

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Bibliographic Details
Published in:Archives of microbiology 2022-08, Vol.204 (8), p.464-464, Article 464
Main Authors: Başkan, Ceren, Sırıken, Belgin, Tüfekci, Enis Fuat, Kılınç, Çetin, Ertürk, Ömer, Erol, İrfan
Format: Article
Language:English
Subjects:
Antibiotics
Biochemistry
Biofilms
Biomedical and Life Sciences
Biotechnology
Carbapenems
Cell Biology
Ecology
Genes
Life Sciences
Microbial Ecology
Microbiology
OprL gene
Original Paper
Pathogenesis
Pseudomonas aeruginosa
Quorum sensing
Slime
Statistical analysis
Virulence
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Summary:Carbapenems are the most effective agents for treating clinical P. aeruginosa (PsA) infections. During an infection, a quorum-sensing (QS) system and its regulating virulence genes have a great role. The aim of the study was to detect the presence of a las and rhl QS system and related virulence genes, biofilm formation and a class 1 (Cls1) integron. A total of 52 carbapenem-resistant PsA (CRPsA) isolates obtained from Kastamonu, Turkey was analyzed. For the isolation and identification of CRPsA isolates, a conventional culture method, an automated VITEK-2 compact system, and oprL gene-based molecular technique were applied. The two QS system genes were detected in 51 (98.1%), and co-existed of four two QS system genes ( lasI / R and rhIl/R genes) were determined in 41 (78.8%) of the isolates. algD, lasB, toxA and aprA genes were detected in between 46.1 and 88.5%, and co-existence of four two QS system genes with four virulence genes were detected in 40.4% of the isolates. Biofilm formation using microtiter plate assay and slime production using Congo Red Agar and Cls1 integron were determined in 84.6%, 67.3% and 51.9% of the isolates, respectively. According to statistical analyses results, there was a significant positive correlation ( p  
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-022-03061-y