Synthesis and Characterization of Tween-20 Capped Biosynthesized Silver Nanoparticles for Anticancer and Antimicrobial Property

• Published in: Applied biochemistry and biotechnology 2023-04, Vol.195 (4), p.2282-2293
• Main Authors: Nithya, Periakaruppan, Raghunathan, Sathya, Prabakaran, Muthusamy, Antony, Susaimanickam Arul, MubarakAli, Davoodbasha
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Infective Agents - pharmacology; Antibacterial activity; Anticancer properties; Antimicrobial agents; Bacteria; Biochemistry; Biosynthesis; Biotechnology; Bone cancer; Chemistry; Chemistry and Materials Science; Crystallites; Crystals; Cytotoxicity; Electron diffraction; Epidermis; Fourier transforms; Fruits; Green Chemistry Technology; Infrared spectrophotometers; Infrared spectroscopy; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Nanoparticles; Original Article; Osteosarcoma; Pathogens; Physiochemistry; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polysorbates; Scanning transmission electron microscopy; Silver; Silver - chemistry; Silver - pharmacology; Spectrophotometry; Spectroscopy, Fourier Transform Infrared; Staphylococcus; Transmission electron microscopy; X ray powder diffraction; X-Ray Diffraction; X-ray spectroscopy
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-022-04069-2

The Vitrus vinifera fruit extract was used to make silver nanoparticles (AgNPs) utilizing a green chemical technique. The biosynthesized Tween-20/ Vitrus vinifera -AgNPs were observed by UV–Vis spectrophotometry. Fourier transform infrared spectroscopy, scanning transmission electron microscopy, selected area electron diffraction, and energy-dispersive X-ray spectroscopy were used to characterize the physiochemical properties. The spherical form of AgNPs was confirmed by transmission electron microscopy. The peaks in the Tween-20/ Vitrus vinifera -AgNPs have an average crystallite size that is found to be 46 nm according to powder X-ray diffraction examination. Biosynthesized AgNPs had a significant effect on bone osteosarcoma MG63 cells with 55% inhibition, respectively, using MTT assay. The effective dangerous concentration of Tween-20/ Vitrus vinifera with AgNP nanoparticles was less harmful to MG63 cells. The results of antibacterial activity showed that Tween-20/ Vitrus vinifera -AgNPs effectively inhibited Eggerthella lenta and Staphylococcus epidermis bacteria.