OaAEP1-mediated PNA-protein conjugation enables erasable imaging of membrane proteins

We report the use of a protein ligase to covalently ligate a protein to a peptide nucleic acid (PNA). The rapid ligation demands only an N-terminal GL dipeptide in the target protein and a C-terminal NGL tripeptide in the PNA. We demonstrate the versatility of this approach by attaching a PNA strand...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2022-07, Vol.58 (60), p.8448-8451
Main Authors: Lu, Zhangwei, Liu, Yutong, Deng, Yibing, Jia, Bin, Ding, Xuan, Zheng, Peng, Li, Zhe
Format: Article
Language:English
Subjects:
Cell membranes
Conjugation
Nucleic acids
Proteins
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Summary:We report the use of a protein ligase to covalently ligate a protein to a peptide nucleic acid (PNA). The rapid ligation demands only an N-terminal GL dipeptide in the target protein and a C-terminal NGL tripeptide in the PNA. We demonstrate the versatility of this approach by attaching a PNA strand to three different proteins. Lastly, we show that erasable imaging of EGFR on HEK293 cell membranes is achieved with DNA origami nanostructures and toehold-mediated strand displacement.
ISSN:1359-7345
1364-548X
DOI:10.1039/d2cc02153f