Effect of Excessive Serotonin on Pharmacokinetics of Cephalexin after Oral Administration: Studies with Serotonin-Excessive Model Rats

• Published in: Pharmaceutical research 2022-09, Vol.39 (9), p.2163-2178
• Main Authors: Nakashima, Shun, Iwamoto, Takeharu, Takanashi, Masashi, Ogawara, Ken-ichi, Maruyama, Masato, Higaki, Kazutaka
• Format: Article
• Language: English
• Subjects: Antibiotics; Antipyrine; Bioavailability; Biochemistry; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Cephalexin; Dextran; Electrical resistivity; Epithelial cells; Intestine; Kidneys; Medical Law; Membrane permeability; Mucosa; Oral administration; Original Research Article; Permeability; Pharmacokinetics; Pharmacology/Toxicology; Pharmacy; Phenols; Physiological aspects; Serotonin
• ISSN: 0724-8741; 1573-904X
• DOI: 10.1007/s11095-022-03325-8

Purpose Serotonin (5-HT) is important for gastrointestinal functions, but its role in drug absorption remains to be clarified. Therefore, the pharmacokinetics and oral absorption of cephalexin (CEX) were examined under 5-HT-excessive condition to understand the role of 5-HT. Methods 5-HT-excessive rats were prepared by multiple intraperitoneal dosing of 5-HT and clorgyline, an inhibitor for 5-HT metabolism, and utilized to examine the pharmacokinetics, absorption behavior and the intestinal permeability for CEX. Results Higher levels of 5-HT in brain, plasma and small intestines were recognized in 5-HT-excessive rats, where the oral bioavailability of CEX was significantly enhanced. The intestinal mucosal transport via passive diffusion of CEX was significantly increased, while its transport via PEPT1 was markedly decreased specifically in the jejunal segment, which was supported by the decrease in PEPT1 expression on brush border membrane (BBM) of intestinal epithelial cells. Since no change in antipyrine permeability and significant increase in FITC dextran-4 permeability were observed in 5-HT-excessive rats, the enhanced permeability for CEX would be attributed to the opening of tight junction, which was supported by the significant decrease in transmucosal electrical resistance. In 5-HT-excessive rats, furthermore, total body clearance of CEX tended to be larger and the decrease in PEPT2 expression on BBM in kidneys was suggested to be one of the reasons for it. Conclusions 5-HT-excessive condition enhanced the oral bioavailability of CEX in rats, which would be attributed to the enhanced permeability across the intestinal mucosa via passive diffusion through the paracellular route even though the transport via PEPT1 was decreased.