Schistosoma mansoni infection decreases IL-33-mRNA expression and increases CXCL9 and CXCL10 production by peripheral blood cells

• Published in: Medical microbiology and immunology 2022-08, Vol.211 (4), p.211-218
• Main Authors: do Nascimento, Wheverton Ricardo Correia, Nóbrega, Cassia Giselle de Oliveira, Fernandes, Erica de Souza, Santos, Patrícia d’Emery Alves, Melo, Fábio Lopes, Albuquerque, Mônica Camelo Pessôa de Azevedo, de Lorena, Virgínia Maria Barros, Costa, Vláudia Maria Assis, Barbosa, Constança Clara Gayoso Simões, de Souza, Valdênia Maria Oliveira
• Format: Article
• Language: English
• Subjects: Antigens; Biomedical and Life Sciences; Biomedicine; Blood cells; Chemokines; CXCL10 protein; Cytokines; Fibrosis; Gene expression; Immunology; Immunoregulation; Interleukin 10; Interleukin 2; Interleukin 4; Interleukin 6; Lymphocytes T; Medical Microbiology; Original Investigation; Peripheral blood; Schistosoma mansoni; Schistosomiasis; Tumor necrosis factor-α; Virology; γ-Interferon
• ISSN: 0300-8584; 1432-1831
• DOI: 10.1007/s00430-022-00745-6

Schistosoma mansoni infections, particularly egg antigens, induce Th2-dominant granulomatous responses accompanied by remarkable immunoregulatory mechanisms that avoid intense fibrosis. Interleukin (IL)-33 is a cytokine that stimulates the early activation of Th2 responses, and its soluble ST2 receptor (sST2) avoids granulomatous response, as well as CXCL9 and CXCL10 chemokines that have antifibrotic activity. However, in schistosomiasis, these molecules have not been suitably studied. Therefore, this study aimed to measure IL-33 and sST2 RNA, cytokines, and chemokines in peripheral blood cultures from individuals living in schistosomiasis-endemic areas. Peripheral blood cells from individuals with S. mansoni ( n  = 34) and non-infected individuals ( n  = 31) were cultured under mitogen stimulation. Supernatant chemokines and cytokines were evaluated using a cytometric bead array, and IL-33 and sST2 mRNA expression was measured using qPCR. Infected individuals showed higher levels of CXCL8, CXCL9, CXCL10, IFN-γ, TNF-α, IL-6, IL-2, IL-4, and IL-10; there was a lower expression of IL-33 mRNA and similar expression of sST2mRNA in infected than non-infected individuals. In conclusion, for the first time, we demonstrated lower IL-33mRNA expression and high levels of the antifibrotic chemokines CXCL9 and CXCL10 in schistosomiasis mansoni , which could control exacerbations of the disease in individuals from endemic areas.