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Upregulation of Hevin contributes to postoperative pain hypersensitivity by inducing neurexin1β/neuroligin1-mediated synaptic targeting of GluA1-containing AMPA receptors in rat dorsal horn

[Display omitted] •Incision enhanced Hevin level and neurexin1β/neuroligin1 interaction in dorsal horn.•Intrathecal Hevin siRNA inhibited postoperative pain hypersensitivity.•Intrathecal Hevin siRNA reduced incision-induced neurexin1β/neuroligin1 interaction.•Intrathecal Hevin siRNA reduced synaptic...

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Published in:Brain research 2022-10, Vol.1792, p.148004-148004, Article 148004
Main Authors: Kang, Yi, Xue, Jianjun, Zheng, Junwei, Liang, Jinghan, Cai, Chenghui, Wang, Yun
Format: Article
Language:English
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Summary:[Display omitted] •Incision enhanced Hevin level and neurexin1β/neuroligin1 interaction in dorsal horn.•Intrathecal Hevin siRNA inhibited postoperative pain hypersensitivity.•Intrathecal Hevin siRNA reduced incision-induced neurexin1β/neuroligin1 interaction.•Intrathecal Hevin siRNA reduced synaptic target of AMPA receptor GluA1 subunit. The astrocytes-secreted active molecule, Hevin considerably contributes in the transsynaptic bridge of neurexin1β/neuligin1 in excitatory synapse. Previous studies have demonstrated that activity-dependent synaptic recruitment of spinal neuroligin1 and GluA1-containing AMPA receptors (AMPARs) is involved in incisional, inflammatory and neuropathic pain. Here, we hypothesized that Hevin induced postoperative pain hypersensitivity by enhancing the neurexin1β/neuroligin1-mediated synaptic targeting of GluA1-containing AMPARs in spinal dorsal horns (DH). Our results showed that plantar incision induced significant postoperative pain behavior, which was described by the cumulative pain scores. At 1 d and 3 d post-incision, Hevin expression was considerably elevated in ipsilateral DHs, although it recovered to baseline value at 5 d following the incision. At 1 d post plantar incision, the neurexin1β/neuroligin1 interactions significantly increased in ipsilateral DHs in rats subjected to incision when compared with those in control rats. Intrathecal pretreatments of small interference RNA targeting Hevin substantially suppressed postoperative pain hypersensitivity and reduced the neurexin1β/neurolgin1 interaction as well as the synaptic targeting of GluA1 in ipsilateral spinal DHs. These data suggest that Hevin induced postoperative pain hypersensitivity by enhancing the neurexin1β/neuroligin1 interaction and subsequent synaptic targeting of GluA1-containing AMPARs in ipsilateral spinal DHs. It provides new insights into the role of Hevin-mediated trans-synaptic regulation in postoperative pain hypersensitivity, which would help develop a novel therapeutic strategy.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2022.148004