Preparation of catalytically active enzyme thin film by alternate deposition of horseradish peroxidase and bipolar quaternary ammonium on solid surface

Multilayered assemblies of horseradish peroxidase (HRP) and bipolar quaternary ammonium salt (NC 6BPC 6N) were fabricated successfully by alternate deposition technique on the surface of quartz slides and gold electrode. The assembly process was monitored by ultraviolet–visible spectra on quartz sli...

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Bibliographic Details
Published in:Materials chemistry and physics 2001-08, Vol.71 (1), p.23-27
Main Authors: Cui, Ai-li, Tang, Jun, Li, Wenjiang, Wang, Zichen, Sun, Changqing, Zhao, Muyu
Format: Article
Language:English
Subjects:
Condensed matter: structure, mechanical and thermal properties
Enzyme electrode
Exact sciences and technology
Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties
Methylene green
Multilayer films
Physics
Self-assembly
Surfaces and interfaces
thin films and whiskers (structure and nonelectronic properties)
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Summary:Multilayered assemblies of horseradish peroxidase (HRP) and bipolar quaternary ammonium salt (NC 6BPC 6N) were fabricated successfully by alternate deposition technique on the surface of quartz slides and gold electrode. The assembly process was monitored by ultraviolet–visible spectra on quartz slides. Cyclic voltammetry indicated that the gold electrode fabricated in this way had the function of reduction of hydrogen peroxide, in which methylene green transferred electrons successfully between the immobilized HRP and the electrode surface.
ISSN:0254-0584
1879-3312
DOI:10.1016/S0254-0584(00)00527-7