Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flumetralin in orange

Residues of flumetralin, a dinitroaniline herbicide, in foods pose a health risk to humans. In this study, we produced a sensitive monoclonal antibody (mAb) against flumetralin and developed a gold nanoparticle-based lateral flow immunoassay (LFIA) strip for screening flumetralin in orange. The subt...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 2022-08, Vol.147 (16), p.3684-3691
Main Authors: Xu, Xinxin, Lin, Lu, Kuang, Hua, Liu, Liqiang, Xu, Liguang, Xu, Chuanlai
Format: Article
Language:English
Subjects:
Gold
Herbicides
Immunoassay
Monoclonal antibodies
Nanoparticles
Strip
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Residues of flumetralin, a dinitroaniline herbicide, in foods pose a health risk to humans. In this study, we produced a sensitive monoclonal antibody (mAb) against flumetralin and developed a gold nanoparticle-based lateral flow immunoassay (LFIA) strip for screening flumetralin in orange. The subtype of mAb 3F5 was IgG1 and had a half-maximal inhibitory concentration of 9.43 ng mL −1 . The cross-reactivity assay indicated that mAb had good specificity. The LFIA strip had a visual limit of detection of 200 ng g −1 in orange. We obtained results with the naked eye within 15 min. The calculated LOD of the strip in orange was 33.26 ng g −1 , with a linear detection of 80.72-1672.10 ng g −1 . The strip had recovery rates ranging from 98.4% to 110.1%. Therefore, our developed method is an effective and reliable tool for the rapid detection of flumetralin in orange. A sensitive monoclonal antibody (mAb) against flumetralin was produced and a gold nanoparticle-based lateral flow immunoassay (LFIA) strip was developed for screening flumetralin in orange.
ISSN:0003-2654
1364-5528
DOI:10.1039/d2an00899h