Overexpression of ZmEREBP60 enhances drought tolerance in maize

Apetala2/ethylene response factor (AP2/ERF) family of transcription factors plays important roles in plant development and stress responses. However, few members of this family have been functionally and mechanistically characterised in maize. In this study, we characterised a member of the AP2/ERF...

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Published in:Journal of plant physiology 2022-08, Vol.275, p.153763-153763, Article 153763
Main Authors: Zhu, Yeqing, Liu, Yue, Zhou, Kaiming, Tian, Congyan, Aslam, Muhammad, Zhang, Binglin, Liu, Weijuan, Zou, Huawen
Format: Article
Language:English
Subjects:
Abscisic acid
Amino acid sequence
Amino acids
Catabolism
Corn
Drought resistance
Drought stress
Gene expression
Hydrogen peroxide
Maize (Zea mays L.)
Nucleotide sequence
Phylogeny
Signal transduction
Transcription factors
Transcriptome analysis
Transcriptomes
Water deprivation
ZmEREBP60
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Summary:Apetala2/ethylene response factor (AP2/ERF) family of transcription factors plays important roles in plant development and stress responses. However, few members of this family have been functionally and mechanistically characterised in maize. In this study, we characterised a member of the AP2/ERF transcription factor family, ZmEREBP60 from maize. Amino acid sequence alignment and phylogenetic analysis showed that ZmEREBP60 belongs to cluster I of the AP2/ERF family. qRT-PCR analysis indicated that ZmEREBP60 expression was highly induced by drought in the roots, coleoptiles, and leaves. Subcellular localisation analysis revealed that ZmEREBP60 was localised in the nucleus. Moreover, overexpression of ZmEREBP60 enhanced tolerance to drought stress while alleviating the drought-induced increase in H2O2 accumulation and malondialdehyde content in transgenic lines. Transcriptome analysis showed that ZmEREBP60 regulates the expression of genes involved in H2O2 catabolism, water deprivation response, and abscisic acid signalling pathway. Collectively, as a new member of the AP2/ERF transcription factor family in maize, ZmEREBP60 is a positive regulator of plant drought response.
ISSN:0176-1617
1618-1328
DOI:10.1016/j.jplph.2022.153763