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Tumor cell-specific retention of photosensitizers determines the outcome of photodynamic therapy for head and neck cancer

Pheophorbide-based photosensitizers have demonstrated tumor cell-specific retention. The lead compound 3-[1′-hexyloxyethyl]-2-devinylpyropheophorbide-a (HPPH) in a clinical trial for photodynamic therapy of head and neck cancer lesions indicated a complete response in 80% of patients. The question a...

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Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2022-09, Vol.234, p.112513-112513, Article 112513
Main Authors: Tracy, Erin C., Bowman, Mary-Jo, Pandey, Ravindra K., Baumann, Heinz
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description Pheophorbide-based photosensitizers have demonstrated tumor cell-specific retention. The lead compound 3-[1′-hexyloxyethyl]-2-devinylpyropheophorbide-a (HPPH) in a clinical trial for photodynamic therapy of head and neck cancer lesions indicated a complete response in 80% of patients. The question arises whether the partial response in 20% of patients is due to inefficient retention of photosensitizers by tumor cells and, if so, can the photosensitizer preference of individual cancer cases be identified prior to photodynamic therapy. This study determined the specificity of head and neck cancer cells and tumor tissues for the uptake and retention of diffusible pheophorbides differing in peripheral groups on the macrocycle that contribute to cellular binding. The relationship between photosensitizer level and light-mediated photoreaction was characterized to identify markers for predicting the effectiveness of photodynamic therapy in situ. The experimental models were stromal and epithelial cells isolated from head and neck tumor samples and integrated into monotypic tissue cultures, reconstituted three-dimensional co-cultures, and xenografts. Tumor cell-specific photosensitizer retention patterns were identified, and a procedure was developed to allow the diagnostic evaluation of HPPH binding by tumor cells in individual cancer cases. The findings of this study may assist in designing conditions for photosensitizer application and photodynamic therapy of head and neck cancer lesions optimized for each patient's case. HPPH binding to tumor cells in a cryosection of a patient's larynx sample (40X). [Display omitted] •Uptake of chlorin-based photosensitizers differs greatly among H/N tumor cells•Retention of photosensitizers is increased in dense tumor cell clusters•Biomarkers for the photoreaction predict PDT outcome•Novel technique determines HPPH-binding in tumors and facilitates optimized PDT.
doi_str_mv 10.1016/j.jphotobiol.2022.112513
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B, Biology</title><description>Pheophorbide-based photosensitizers have demonstrated tumor cell-specific retention. The lead compound 3-[1′-hexyloxyethyl]-2-devinylpyropheophorbide-a (HPPH) in a clinical trial for photodynamic therapy of head and neck cancer lesions indicated a complete response in 80% of patients. The question arises whether the partial response in 20% of patients is due to inefficient retention of photosensitizers by tumor cells and, if so, can the photosensitizer preference of individual cancer cases be identified prior to photodynamic therapy. This study determined the specificity of head and neck cancer cells and tumor tissues for the uptake and retention of diffusible pheophorbides differing in peripheral groups on the macrocycle that contribute to cellular binding. The relationship between photosensitizer level and light-mediated photoreaction was characterized to identify markers for predicting the effectiveness of photodynamic therapy in situ. 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subjects Binding
Cancer
Cancer cell models
Epithelial cells
Epithelium
Head & neck cancer
Lead compounds
Lesions
Patients
PDT biomarker
Pheophorbide
Photodynamic therapy
Photoreaction
Retention
Specific retention
Tumor cells
Tumor tissue analysis
Tumors
Xenografts
Xenotransplantation
title Tumor cell-specific retention of photosensitizers determines the outcome of photodynamic therapy for head and neck cancer
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