Suppression of macrophage-mediated xenogeneic rejection by the ectopic expression of human CD177

Cellular xenogeneic rejection by the innate immune system is a major immunological obstruction that needs to be overcome for the successful clinical use of xenografts. Our focus has been on macrophage-mediated xenogeneic rejection, since suppressing macrophage function has considerable potential for...

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Bibliographic Details
Published in:Transplant immunology 2022-10, Vol.74, p.101663-101663, Article 101663
Main Authors: Kogata, Shuhei, Lo, Pei-Chi, Maeda, Akira, Okamatsu, Chizu, Sato, Kazuki, Yamamoto, Riho, Haneda, Tomoko, Yoneyama, Tomohisa, Toyama, Chiyoshi, Eguchi, Hiroshi, Masahata, Kazunori, Kamiyama, Masafumi, Okuyama, Hiroomi, Miyagawa, Shuji
Format: Article
Language:English
Subjects:
Human CD177
Human CD31
Macrophage
Xenogeneic rejection
Xenotransplantation
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Summary:Cellular xenogeneic rejection by the innate immune system is a major immunological obstruction that needs to be overcome for the successful clinical use of xenografts. Our focus has been on macrophage-mediated xenogeneic rejection, since suppressing macrophage function has considerable potential for practical applications in the area of xenotransplantation. We report herein on an investigation of the suppressive effect of human CD177 (hCD177) against macrophage-mediated xenogeneic rejection. Wild type swine aortic endothelial cell (SEC) and an SEC transfectant with hCD177 (SEC/hCD177) were co-cultured with macrophages, and the degree of cytotoxicity was evaluated by WST-8 assays, and phagocytosis was examined using Calcein-AM labeling methods. The expression of anti/pro-inflammatory cytokines was evaluated by RT-qPCR and the phosphorylation of SHP-1 on macrophages in co-culture was evaluated by Western blotting. The result of cytotoxicity assays indicated that hCD177 suppressed M1 macrophage-mediated xenogeneic rejection (vs. SEC, p 
ISSN:0966-3274
1878-5492
DOI:10.1016/j.trim.2022.101663