In vitro evaluation of a synthetic (Biobrane®) and a biopolymer (Epicite) wound dressing with primary human juvenile and adult fibroblasts after different colonization strategies

• Published in: Annals of anatomy 2022-10, Vol.244, p.151981-151981, Article 151981
• Main Authors: Gögele, Clemens, Schulze-Tanzil, Gundula, Gäbel, Christiane, Breier, Annette, Billner, Moritz, Reichert, Bert, Bodenschatz, Karl
• Format: Article
• Language: English
• Subjects: Biobrane; Burn; Epicite; Murine and primary human dermal fibroblast; Wound dressing
• ISSN: 0940-9602; 1618-0402
• DOI: 10.1016/j.aanat.2022.151981

The three-dimensional [3D] wound dressings Biobrane® and Epicite are used in the wound management. Fibroblasts are important for successful deep wound healing. The direct effect of Biobrane® and Epicite on human fibroblasts, particularly of juvenile individuals, remains unclear. Therefore, this study compared the survival and growth characteristics of juvenile and adult dermal fibroblasts on Biobrane® and Epicite using different culture models. Murine (L929), primary juvenile and adult human fibroblasts were seeded on both materials using two dimensional (2D, slide culture) or 3D culture at the medium-air interface and dynamical rotatory culture. Cell adherence, viability, morphology, actin cytoskeleton architecture and DNA content were monitored. Scanning electron microscopy (SEM) analyses could be only performed from Biobrane®. Permeability of both materials were tested. The majority of all tested fibroblasts species survived on both dressings with no significant differences between 1 and 14 days. Juvenile and adult fibroblasts exerted typical fibroblast morphology with spindle-shaped cell bodies on the materials. SEM visualized morphological differences between murine and human fibroblasts on Biobrane®. Juvenile and adult fibroblasts colonized Biobrane® in rotatory culture after 7 days the most. The Biobrane® rotatory culture of L929 and juvenile fibroblasts showed after 7 days the significantly highest DNA amount. No major gender differences could be observed. Biobrane® had a higher permeability than Epicite. Both wound dressing can be colonized by fibroblasts suggesting their high cytocompatibility. Fibroblast survival and morphology on Biobrane® and Epicite depended on the culture system and the fibroblast source. •Both wound dressing can be colonized by human fibroblasts.•The high cell viability on the dressings underlined cytocompatibility for all fibroblast types tested.•Juvenile and adult fibroblasts showed the highest colonized area on Biobrane in the rotatory culture after 7 days.•No major gender differences of primary human fibroblasts.•Juvenile fibroblasts show higher proliferation on wound dressings than adult fibroblasts.