The pyrethroid insecticide permethrin confers hepatotoxicity through DNA damage and mitochondria‐associated apoptosis induction in rat: Palliative benefits of Fumaria officinalis

• Published in: Journal of biochemical and molecular toxicology 2022-10, Vol.36 (10), p.e23172-n/a
• Main Authors: Aoiadni, Nissaf, Chiab, Nour, Jdidi, Hajer, Gargouri Bouzid, Radhia, El Feki, Abdelfattah, Fetoui, Hamadi, Ghorbel Koubaa, Fatma
• Format: Article
• Language: English
• Subjects: Antioxidants; Apoptosis; Body weight; Calcium ions; Deoxyribonucleic acid; DNA; DNA damage; DNA fragmentation; Ferulic acid; Flavonoids; Fumaria officinalis; Genotoxicity; Hepatotoxicity; Hydrogen peroxide; Insecticides; Lactic acid; Liquid chromatography; Liver; Magnesium; Mitochondria; Mitochondrial DNA; Naringenin; Necrosis; Oral administration; Oxidative stress; Palliation; Permethrin; Pesticides; Phenols; Polyphenols; Pyrethroids; Pyruvic acid; Quercetin; Tannins; Toxicity
• ISSN: 1095-6670; 1099-0461
• DOI: 10.1002/jbt.23172

Permethrin (PER) is a pyrethroid pesticide that is extensively used as an insecticide in world because of its high activity and its low mammalian toxicity. The current study was conducted to investigate the protective action of Fumaria officinalis against PER‐induced liver injury in male rats. However, HPLC‐DAD showed the richness of 6 components in F. officinalis (F) including quercetin, ferulic acid, and naringenin which were the most abundant. Total polyphenols, total flavonoids, and condensed tannins were studied by phytochemical screening. In vitro, antioxidant properties showed that F. officinalis exhibited the highest DPPH radical, FRAP, and H2O2 tests and total antioxidant capacity. Wistar rats were divided into four groups: negative control group (C), positive control group (F) (200 mg F. officinalis/kg BW), PER group (34.05 mg permethrin/kg BW), and PER + F group (34.05 mg permethrin/kg BW and 200 mg F. officinalis/kg BW). Oral administration of PER led to promote a decrease of body weight and Ca2+‐ATPases and Mg2+‐ATPases activities and an increase of plasma C‐reactive protein level, transaminases, and hepatic ϒ‐GT activities as well as hepatic and mitochondrial oxidative stress. An increase in plasma lactate‐to pyruvate ratio and a reduction in complexes enzymes I, III, and IV activities were also observed. In addition, histoarchitecture of liver in PER‐treated rats showed apoptosis and necrosis as confirmed by DNA fragmentation. F. officinalis significantly exerted hepatoprotective effect by modulating hepatic alteration and mitochondrial dysfunction as well as genotoxicity. This effect could be attributed to phenolics compounds such as polyphenols, condensed tannins, and flavonoids. Fumaria officinalis supplementation ameliorates hepatotoxicity and mitochondria dysfunction induced by permethrin