Amphiphilic nano-delivery system based on modified-chitosan and ovalbumin: Delivery and stability in simulated digestion

• Published in: Carbohydrate polymers 2022-10, Vol.294, p.119779-119779, Article 119779
• Main Authors: Li, Lihua, Sun, Xinyu, Zhang, Hui, Dong, Mengna, Wang, Jiao, Zhao, Shuang, Shang, Minghui, Wang, Xin, Zhangsun, Hui, Wang, Li
• Format: Article
• Language: English
• Subjects: Amphiphilic nano-delivery system; Delivery capacity; Gallic acid modified chitosan; Ovalbumin; Stability
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2022.119779

Nano-delivery systems play an important role in the development of nutritional supplements due to their efficient encapsulation and delivery properties for nutrients. Herein, we prepared protein-polysaccharide nanoparticles as a novel amphiphilic nano-delivery system based on gallic acid modified chitosan (GCS) and ovalbumin (OVA) by pH-driven and calcium ion crosslinking. The nanoparticles loaded with hydrophilic riboflavin (Rib) and hydrophobic quercetin (Que) as nutrient models were abbreviated as GCS-OVA-Rib NPs and GCS-OVA-Que NPs, respectively. Their encapsulation efficiencies for Rib and Que. were 66.36 % and 96.61 %, respectively. In addition, GCS-OVA-Rib NPs and GCS-OVA-Que NPs showed antioxidant activity as well as good stability and delivery capacity for Rib and Que. in simulated digestion with release ratios of 78.38 % and 84.15 %, respectively. More importantly, GCS-OVA-Rib/Que. NPs performed good biocompatibility for further applications. Overall, this work provides some useful insights for the design of novel amphiphilic nano-delivery systems based on polysaccharides and proteins. [Display omitted]