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Interferon‐gamma inducible protein 16 and type I interferon receptors expression in experimental apical periodontitis induced in wild‐type mice
Aim The aim of this study was to evaluate the IFI16 and IFN‐α/β receptors expression during the genesis and development of experimental apical periodontitis (AP) in mice teeth. Methodology Apical periodontitis was induced in the lower first molars of 40 C57BL/6 mice. They were divided according to t...
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Published in: | International endodontic journal 2022-10, Vol.55 (10), p.1042-1052 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Aim
The aim of this study was to evaluate the IFI16 and IFN‐α/β receptors expression during the genesis and development of experimental apical periodontitis (AP) in mice teeth.
Methodology
Apical periodontitis was induced in the lower first molars of 40 C57BL/6 mice. They were divided according to the experimental periods 2, 7, 14, 21 and 42 days (n = 8 per group). Five animals were used as a control group (without AP). Specimens were submitted to histological processing for description of the inflammatory process, immunostaining for the presence/absence and localization of IFI16 and IFN‐α/β receptors (qualitative and semi‐quantitative analysis) and tartrate‐resistant acid phosphatase (TRAP) histoenzimology.
Results
The results showed a gradual development of AP over the experimental times. The expression of IFI16 was noticeably more exacerbated in the experimental early period (day 2) whilst the lowest expression was observed in the control group (p = .02). For IFN‐α/β receptors, a higher intensity staining was observed 42 days after AP induction, that was statistically different from the control group (p = .02). In addition, the number of TRAP‐positive cells was higher on the later periods (days 21 and 42; p |
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ISSN: | 0143-2885 1365-2591 |
DOI: | 10.1111/iej.13802 |