Selective reverse micellar extraction of penicillin acylase from E coli

The recovery of penicillin acylase from E coli by a new reverse micellar treatment is described. The results are compared with the cell disruption by ultrasound followed by reverse micellar extraction. The process gave selective extraction of penicillin acylase directly from the periplasmic space of...

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Bibliographic Details
Published in:Journal of chemical technology and biotechnology (1986) 2001-07, Vol.76 (7), p.729-736
Main Authors: Gaikar, Vilas G, Kulkarni, Madhav S
Format: Article
Language:English
Subjects:
AOT
Biological and medical sciences
Biotechnology
Enzyme engineering
Escherichia coli
extraction
Fundamental and applied biological sciences. Psychology
Improved methods for extraction and purification of enzymes
Methods. Procedures. Technologies
penicillin acylase
reverse micelles
ultrasonication
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Summary:The recovery of penicillin acylase from E coli by a new reverse micellar treatment is described. The results are compared with the cell disruption by ultrasound followed by reverse micellar extraction. The process gave selective extraction of penicillin acylase directly from the periplasmic space of E coli without disrupting the cells. Unlike ultrasonication which breaks open the cells entirely, making subsequent processing difficult and expensive, reverse micellar treatment of cells gave a moderate recovery of 60% of enzyme activity in a highly pure form. © 2001 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.444