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A high-resolution colorimetric immunoassay for tyramine detection based on enzyme-enabled growth of gold nanostar coupled with smartphone readout
•Monoclonal antibody specific for tyramine was prepared based on a new hapten design.•Alkaline phosphatase enabled the growth of gold nanostars for multicolor generation.•A multicolor colorimetric immunoassay for tyramine was developed.•Smartphone was used for quantitative detection of tyramine. In...
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Published in: | Food chemistry 2022-12, Vol.396, p.133729-133729, Article 133729 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | •Monoclonal antibody specific for tyramine was prepared based on a new hapten design.•Alkaline phosphatase enabled the growth of gold nanostars for multicolor generation.•A multicolor colorimetric immunoassay for tyramine was developed.•Smartphone was used for quantitative detection of tyramine.
In this work, a specific monoclonal antibody against tyramine was produced based on a new hapten design. Then, we developed a high-resolution multicolor colorimetric immunoassay for tyramine based on this antibody by integrating enzyme-induced multicolor generation with smartphone-assistant signal readout. The multicolor generation is due to the shift of the local surface plasmon resonance band of gold nanostructure controlled by alkaline phosphatase-induced the growth of gold nanostars. Quantitative detection of tyramine was achieved via analyzing the red/blue channel values of assay solution’s image taken by a smartphone with the support of a color recognizer application. The limit of detection of this immunoassay for tyramine detection in beef, pork and yoghurt was 19.7 mg/kg or L. The average recoveries were between 83 % and 103 %., and the results were validated by high performance liquid chromatography to be reliable. Overall, this developed immunoassay provides a promising platform for on-site detection of tyramine. |
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ISSN: | 0308-8146 1873-7072 |
DOI: | 10.1016/j.foodchem.2022.133729 |