High-sodium maltobionate production by immobilized Zymomonas mobilis cells in polyurethane

• Published in: Bioprocess and biosystems engineering 2022-09, Vol.45 (9), p.1465-1476
• Main Authors: de Souza, Roberta Cristina, da Silva, Leonardo Meirelles, Carra, Sabrina, Flores, Maicon, Puton, Bruna Maria, Malvessi, Eloane, Valduga, Eunice, Zeni, Jamile
• Format: Article
• Language: English
• Subjects: Acids; Bioconversion; Biotechnology; Chemistry; Chemistry and Materials Science; Engineering; Environmental Engineering/Biotechnology; Enzymes; Food Science; Glucose; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Isocyanates; Maltose; pH effects; Polyurethane; Polyurethane resins; Research Paper; Silicones; Sodium; Stability analysis; Substrates; Zymomonas mobilis
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-022-02756-2

The purpose of this study was the production of maltobionic acid, in the form of sodium maltobionate, by Z. mobilis cells immobilized in polyurethane. The in situ immobilized system (0.125–0.35 mm) was composed of 7 g polyol, 3.5 g isocyanate, 0.02 g silicone, and 7 g Z. mobilis cell, at the concentration of 210 g/L. The bioconversion of maltose to sodium maltobionate was performed with different cell concentrations (7.0–9.0 g imobilized /L reaction_medium ), temperature (30.54–47.46 °C), pH (5.55–7.25), and substrate concentration (0.7–1.3 mol/L). The stability of the immobilized system was evaluated for 24 h bioconversion cycles and storage of 6 months. The maximum concentration of sodium maltobionate was 648.61 mmol/L in 34.34 h process (8.5 g dry_cell /L reaction_medium ) at 39 °C and pH 6.30. The immobilized system showed stability for 19 successive operational cycles of 24 h bioconversion and 6 months of storage, at 4 °C or 22 °C.