A hemicyanidin-based NIR fluorescent probe for detection of H2S and imaging study in cells and mice

• Published in: Mikrochimica acta (1966) 2022-08, Vol.189 (8), p.291-291, Article 291
• Main Authors: Li, Bai, Wang, Minghui, Gu, Xin, Chen, Jiajia, Yang, Xindi, Liu, Xuejin, Xu, Kuoxi
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Fluorescent indicators; Hydrogen sulfide; Imaging; Intestine; Microengineering; Nanochemistry; Nanotechnology; NMR; Nuclear magnetic resonance; Original Paper
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-022-05374-7

The selective detection of hydrogen sulfide in physiological and pathological processes has gained substantial attention in recent years. However, the real-time detection of hydrogen sulfide remains an elusive goal. In this work, a new type of hemicyanidin-based fluorescent “turn-on” probe NTR-HS (Ex = 680 nm, Em = 760 nm) was developed to detected H 2 S in a very short time (3 min). The fluorescence quantum yield is 0.15 and accompanied with a noticeable color change from violet to blue that can be used to detect H 2 S in the range 1.04 × 10 −7 –4 × 10 −5  M with a limit of detection of 1.04 x 10 -7 M. The NTR-HS probe was also used for imaging of endogenous hydrogen sulfide and mitochondrial localization in HCT116 and HeLa cells. The detection mechanism was studied through fluorescence, UV–Vis, NMR, and mass analysis. Notably, the probe was successfully used to imaging H 2 S in mice and locating hydrogen sulfide in the large intestine of mice. Graphical abstract