Virulence gene profile and antimicrobial resistance of non-typeable Streptococcus suis isolated from clinically healthy and diseased pigs from North East India

The present study was conducted to investigate the virulence gene profile and antimicrobial resistance of non-typeable Streptococcus suis isolates circulating in pigs of North East India. Fifty-two non-typeable S. suis isolates from clinically healthy and diseased pigs were screened by using PCR for...

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Published in:Folia microbiologica 2022-12, Vol.67 (6), p.947-953
Main Authors: Rajkhowa, Swaraj, Hussain, Isfaqul
Format: Article
Language:English
Subjects:
Antimicrobial agents
Antimicrobial resistance
Applied Microbiology
Arginine deiminase
Biomedical and Life Sciences
Drug resistance
Environmental Engineering/Biotechnology
Glutamate dehydrogenase
Immunology
Life Sciences
Microbiology
Multidrug resistance
Original Article
Rabbits
Streptococcus infections
Streptococcus suis
Suilysin
Sulfonamides
Swine
Tetracyclines
Virulence
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description The present study was conducted to investigate the virulence gene profile and antimicrobial resistance of non-typeable Streptococcus suis isolates circulating in pigs of North East India. Fifty-two non-typeable S. suis isolates from clinically healthy and diseased pigs were screened by using PCR for the presence of the muramidase-released protein ( mrp ), extracellular factor ( epf ), hemolysin suilysin ( sly ), arginine deiminase ( arcA ), and glutamate dehydrogenase ( gdh ) genes. Five different virulence gene profiles were observed and the most predominant virulence gene profile found in healthy pigs was mrp − + sly − + arcA − + gdh + + epf − whereas the most predominant virulence gene profile recorded in diseased pigs was mrp + + sly − + arcA + + gdh + + epf − . Significantly lower carrier rate of mrp + + sly − + arcA + + gdh + + epf − virulence gene profile was observed among the isolates from healthy pigs compared to those from diseased pigs ( P < 0.05). Antimicrobial resistance patterns of the S. suis isolates revealed fourteen resistance groups (R1 to R14) where 88.46% isolates showed multi-drug resistance. The most predominant resistance pattern observed was CD-COT-E-TE. This is perhaps the first study reporting virulence gene profile and antimicrobial resistance of non-typeable S. suis isolates from pigs in North East India. The occurrence of relatively high levels of resistance of S. suis to some antimicrobials (e.g. macrolides, tetracyclines, and sulphonamides) as observed in the present study may represent a human health concern.
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1874-9356
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source Springer Nature
subjects Antimicrobial agents
Antimicrobial resistance
Applied Microbiology
Arginine deiminase
Biomedical and Life Sciences
Drug resistance
Environmental Engineering/Biotechnology
Glutamate dehydrogenase
Immunology
Life Sciences
Microbiology
Multidrug resistance
Original Article
Rabbits
Streptococcus infections
Streptococcus suis
Suilysin
Sulfonamides
Swine
Tetracyclines
Virulence
title Virulence gene profile and antimicrobial resistance of non-typeable Streptococcus suis isolated from clinically healthy and diseased pigs from North East India
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