Comparison of bacterial diversity, root exudates and soil enzymatic activities in the rhizosphere of AVP1‐transgenic and nontransgenic wheat (Triticum aestivum L.)

• Published in: Journal of applied microbiology 2022-11, Vol.133 (5), p.3094-3112
• Main Authors: Arshad, Muhammad, Naqqash, Tahir, Tahir, Muhammad, Leveau, Johan H., Zaheer, Ahmad, Tahira, Syeda Anjum, Saeed, Nasir Ahmad, Asad, Shaheen, Sajid, Muhammad
• Format: Article
• Language: English
• Subjects: Bacteria; bacterial diversity; Copy number; Deoxyribonucleic acid; DNA; Drought; Enzymatic activity; Enzymes; Exudates; Exudation; Gene sequencing; Genera; Host plants; Microbial activity; Microorganisms; NifH gene; Nucleotide sequence; Organic acids; Phenoloxidase; Phenols; Plants (botany); Pyrophosphatase; Rhizosphere; Risk assessment; Roots; rRNA 16S; Sequence analysis; Side effects; Soil conditions; Soil ecology; Soil environment; soil enzymes; Soil microorganisms; Soil solution; Soils; Sugar; Transgenes; Transgenic plants; Triticum aestivum; Urease; Wheat
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.15751

Aims Soil microbial communities are among the most diverse communities that might be affected due to transgenic crops. Therefore, risk assessment studies on transgenes are essentially required as any adverse effects may depend not only on the specific gene and crop involved but also on soil conditions. Methods and results The present study deals with the comparison of bacterial populations, root exudates and activities of soil enzymes in nontransgenic and AVP1‐transgenic wheat rhizosphere, overexpressing vacuolar H + pyrophosphatase for salinity and drought stress tolerance. Amounts of organic acids and sugars produced as root exudates and activities of dehydrogenase, phosphatase and protease enzymes in soil solution showed no significant differences in AVP1‐transgenic and nontransgenic wheat rhizosphere, except for urease and phenol oxidase activities. The higher copy number of nifH gene showed the abundance of nitrogen‐fixing bacteria in the rhizosphere of AVP1‐transgenic wheat compared with nontransgenic wheat. nifH gene sequence analysis indicated the common diazotrophic genera Azospirillum, Bradyrhizobium, Rhizobium and Pseudomonas in AVP1‐transgenic and nontransgenic wheat except for Zoogloea detected only in nontransgenic wheat. Using 454‐pyrosequencing of 16S rRNA gene from soil DNA, a total of 156, 282 sequences of 18 phyla were obtained, which represented bacterial (128,006), Archeal (7928) and unclassified (21,568) sequences. Proteobacteria, Crenarchaeota and Firmicutes were the most abundant phyla in the transgenic and nontransgenic wheat rhizosphere. Further comparison of different taxonomic units at the genus level showed similar distribution in transgenic and nontransgenic wheat rhizospheres. Conclusion We conclude that the AVP1 gene in transgenic wheat has no apparent adverse effects on the soil environment and different bacterial communities. However, the bacterial community depends on several other factors, not only genetic composition of the host plants. Significance of the study The present research supports introduction and cultivation of transgenic plants in agricultural systems without any adverse effects on indigenous bacterial communities and soil ecosystems.