Phytochemical profile, in vivo anti-inflammatory and wound healing activities of the aqueous extract from aerial parts of Cistus ladanifer L

• Published in: Journal of pharmaceutical and biomedical analysis 2022-09, Vol.219, p.114960-114960, Article 114960
• Main Authors: Adadi, Imad, Ayadi, Rachida El, Bentayeb, Amar, Aaziz, Hicham, Bouymajane, Aziz, Altemimi, Ammar B., Cacciola, Francesco, Ibaoui, Hamid El
• Format: Article
• Language: English
• Subjects: Anti-inflammatory; Cistus ladanifer L; Cream; Rats; UHPLC-ESI-MS/MS; Wound healing
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2022.114960

Cistus ladanifer L. is a Mediterranean shrub, well recognized for its many pharmacological properties. The present study aimed to investigate the phytochemical profile, the in vivo anti-inflammatory and the wound healing activities of the aqueous extract from its aerial parts (AECL). The in vivo anti-inflammatory and the wound healing properties were evaluated by carrageenan-induced paw edema (CAECL) and burn wound in rats, respectively. The results showed that six compounds belonging to flavonoids and tannins were identified in the AECL extract. Moreover, the AECL administrated orally at a dose of 500 mg/kg significantly reduced edema with a percentage of 72 % in terms of inflammation inhibition, using indomethacin as reference drug. On the other hand, when administrated topically, both doses of CAECL cream (5 % and 10 %) manifested a significant anti-inflammatory action using diclofenac as reference drug with a percentage reduction of inflammation that reached 85 % (CAECL-10). As far as wound healing is concerned, the CAECL showed higher effect at a dose of 10 % (CAECL-10) producing a wound contraction of 96 %, similar to that of the reference drug. The findings of the present study indicate that the aqueous extract from aerial parts of Cistus ladanifer L. display strongly anti-inflammatory and wound healing effects to be advantageously used for medical purposes. •Six compounds were identified in the aqueous extract from aerial parts of Cistus ladanifer L (AECL).•AECL administrated orally in rat at a dose of 500 mg/kg significantly reduced edema with percentage inhibition of 72 %.•The CAECL-10 exhibited strong anti-inflammatory and wound healing effects (reduction and wound contraction: 85% and 96%).•The extract from aerial parts of Cistus ladanifer L. could be exploited in the treatment of inflammation and wound healing.