Sensing Base Modifications in Non-Canonically Folded DNA with an Optimized Host:Guest Sensing Array

An arrayed host:guest fluorescence sensor system can discriminate DNA G-quadruplex structures that differ only in the presence of single oxidation or methylation modification in the guanine base. These small modifications make subtle changes to G4 folding that are often not detectable by CD but indu...

Full description

Saved in:
Bibliographic Details
Published in:ACS sensors 2022-08, Vol.7 (8), p.2164-2169
Main Authors: Chen, Junyi, Hickey, Briana L., Gao, Ziting, Raz, Alexie Andrea P., Hooley, Richard J., Zhong, Wenwan
Format: Article
Language:English
Subjects:
DNA - chemistry
Fluorescence
G-Quadruplexes
Guanine - chemistry
Oxidation-Reduction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An arrayed host:guest fluorescence sensor system can discriminate DNA G-quadruplex structures that differ only in the presence of single oxidation or methylation modification in the guanine base. These small modifications make subtle changes to G4 folding that are often not detectable by CD but induce differential fluorescence responses in the array. The sensing is functional in diluted serum and is capable of distinguishing individual modifications in DNA mixtures, providing a powerful method of detecting folding changes caused by DNA damage.
ISSN:2379-3694
2379-3694
DOI:10.1021/acssensors.2c00839