Simultaneous Monitoring of the Adenosine Triphosphate Levels in the Cytoplasm and Nucleus of a Single Cell with a Single Nanowire-Based Fluorescent Biosensor

Simultaneous monitoring of the ATP levels at various sites of a single cell is crucial for revealing the ATP-related processes and diseases. In this work, we rationally fabricated single nanowire-based fluorescence biosensors, by which the ATP levels of the cytoplasm and nucleus in a single cell can...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2022-08, Vol.94 (34), p.11813-11820
Main Authors: Mei, Mingliang, Mu, Lixuan, Wang, Yuan, Liang, Sen, Zhao, Qiaowen, Huang, Lushan, She, Guangwei, Shi, Wensheng
Format: Article
Language:English
Subjects:
Adenosine triphosphate
Apoptosis
ATP
Biosensors
Calcium ions
Chemistry
Cisplatin
Cytoplasm
Fluorescence
Monitoring
Nanotechnology
Nanowires
Nuclei (cytology)
Oligomycin
Spatial discrimination
Spatial resolution
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Summary:Simultaneous monitoring of the ATP levels at various sites of a single cell is crucial for revealing the ATP-related processes and diseases. In this work, we rationally fabricated single nanowire-based fluorescence biosensors, by which the ATP levels of the cytoplasm and nucleus in a single cell can be simultaneously monitored with a high spatial resolution. Utilizing the as-fabricated single nanowire biosensor, we demonstrated that the ATP levels of the cytoplasm were around 20–30% lower than that of the nucleus in both L929 and HeLa cells. Observing the ATP fluctuation of the cytoplasm and nucleus of the L929 and HeLa cells stimulated by Ca2+, oligomycin, or under cisplatin-induced apoptosis, we found that the ATP levels at two cellular sites exhibited discriminative changes, revealing the different mechanisms of the ATP at these two cellular sites in response to the stimulations.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.2c02030