In vitro antioxidant and anti-inflammatory activity of an oleuropein-enriched extract obtained from olives leaves on BME-UV1 cells

In this study, for the first time was evaluated the antioxidant and anti-inflammatory effect of an Oleuropein-enriched extract (OleE) on bovine mammary epithelial cell line (BME-UV1). OleE was obtained from olives leaves and characterized by HPLC and NMR analysis. Cell viability test indicated that...

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Bibliographic Details
Published in:Natural product research 2023-05, Vol.37 (10), p.1725-1729
Main Authors: Basiricò, Loredana, Mastrogiovanni, Fabio, Lacetera, Nicola, Santi, Luca, Bernini, Roberta, Bernabucci, Umberto
Format: Article
Language:English
Subjects:
Animals
Anti-inflammatory agents
Anti-Inflammatory Agents - pharmacology
Antioxidants
Antioxidants - metabolism
Antioxidants - pharmacology
Cattle
Cell Line
Cell viability
Cytotoxicity
Dairy cattle
Epithelial Cells
Epithelium
Feed supplements
Gene expression
Hydrogen peroxide
In vivo methods and tests
Inflammation
Interleukin 10
Liquid chromatography
Mammary gland
NMR
Nuclear magnetic resonance
Olea
Oleaceae
Oleuropein
Olives
Oxidative stress
Plant Extracts - metabolism
Plant Extracts - pharmacology
Reactive Oxygen Species - metabolism
Reduction
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Summary:In this study, for the first time was evaluated the antioxidant and anti-inflammatory effect of an Oleuropein-enriched extract (OleE) on bovine mammary epithelial cell line (BME-UV1). OleE was obtained from olives leaves and characterized by HPLC and NMR analysis. Cell viability test indicated that OleE at concentrations of 7.8 up to 250 μg/mL did not exert cytotoxic effect. At concentration of 31.2 up to 250 μg/mL, a dose dependent reduction of ROS production induced by hydrogen peroxide was observed. In addition, OleE at 62.5, 125 and 250 μg/mL showed a dose-dependent reduction in gene expression of , , and after exposure to LPS. The downregulation of ROS production and cytokines expression in BME-UV1 by OleE confirmed the antioxidant and anti-inflammatory properties. experiments will be necessary for future applications of OleE as natural feed supplement in dairy cattle to reduce incidence of oxidative stress in peripartal period.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2022.2106485