The presence of bioactive peptides in hydrolysates prepared from processing waste of sardine (Sardina pilchardus)

A proteolytic enzyme, Alcalase®, was used to produce partly digested proteins from cooked wastes of sardine (Sardina pilchardus). The presence of biologically active molecules was investigated in these hydrolysates prepared under various conditions of time and enzyme/substrate ratio. By means of rad...

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Bibliographic Details
Published in:Journal of the science of food and agriculture 2001-09, Vol.81 (11), p.1120-1125
Main Authors: Ravallec-Plé, Rozenn, Charlot, Caroline, Pires, Carla, Braga, Vera, Batista, Irineu, Van Wormhoudt, Alain, Le Gal, Yves, Fouchereau-Péron, Martine
Format: Article
Language:English
Subjects:
Alcalase
Biological and medical sciences
CGRP
Fish and seafood industries
Food industries
Fundamental and applied biological sciences. Psychology
gastrin
growth factor
sardine hydrolysates
Use and upgrading of agricultural and food by-products. Biotechnology
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Summary:A proteolytic enzyme, Alcalase®, was used to produce partly digested proteins from cooked wastes of sardine (Sardina pilchardus). The presence of biologically active molecules was investigated in these hydrolysates prepared under various conditions of time and enzyme/substrate ratio. By means of radioimmunoassays and mitogenic and radioreceptor assays, the presence of molecules related to secretagogue peptides, growth factors and calcitonin gene‐related peptide (CGRP) respectively was detected in the hydrolysates. Exclusion chromatography permits the conclusion that the biological activity of the different fractions is related to their size. © 2001 Society of Chemical Industry
ISSN:0022-5142
1097-0010
DOI:10.1002/jsfa.921