SERS-based biosensor with Raman-active external responsive element for rapid determination of adenosine monophosphate

• Published in: Analytica chimica acta 2022-08, Vol.1221, p.340140-340140, Article 340140
• Main Authors: Zavyalova, Elena, Tikhonova, Daria, Zhdanov, Gleb, Rudakova, Elena, Alferova, Vera, Moiseenko, Andrey, Kamzeeva, Polina, Khrulev, Alexei, Zalevsky, Arthur, Arutyunyan, Alexander, Novikov, Roman, Kukushkin, Vladimir, Aralov, Andrey
• Format: Article
• Language: English
• Subjects: AMP; Aptamer; BHQ; Raman; Sensor; SERS
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2022.340140

Phosphorylated adenosine derivatives are important biological molecules with diverse biological functions connected with the energetic balance of the cell, biosynthesis of cell components and regulation of protein activity. Measurement of these compounds provides information about the cell signalling in the body as well as the quantity of microorganisms in the environment. Surface-enhanced Raman spectroscopy (SERS) is an optical method that provides a unique spectrum of a substance at low concentrations. Specificity and limit of detection of SERS-based sensors can be increased drastically using nucleic acid aptamers and Raman-active dyes, respectively. Here we describe an adenosine monophosphate (AMP) biosensor based on AMP-dependent interaction between the well-known DNA aptamer for AMP and a novel Raman-active dye. The SERS intensity of novel Black Hole Quencher-2 (BHQ-2) derivatives was shown to be proportional to the charge of the molecule indicating electrostatic interactions with negatively charged colloidal silver nanoparticles. The novel derivative of BHQ-2 with two amine groups, BHQ-2-(NH2)2, binds an unpaired guanine stacked between guanine-guanine and guanine-adenine mismatches in DNA aptamer-AMP complex with KD = 26 nM as shown by 1H nuclear magnetic resonance, molecular docking and biolayer interferometry. The aptamer is pre-structured by AMP being folded in the conformation favorable for the interaction with BHQ-2-(NH2)2. This specific mechanism of the interaction allows designing of a SERS-based aptasensor with a limit of detection being as low as 3.4 nM of AMP and the dynamic range of nearly 5 orders – from 3.4 nM to 200 μM. The results illustrate a new approach to biosensors where DNA-interacting ligands act as external responsive elements providing an analyte-dependent SERS signal. [Display omitted] •Novel Raman-active BHQ derivatives with positively charged groups were synthesized.•The derivatives provide SERS spectrum at low-nanomolar concentrations.•BHQ-2-(NH2)2 binds DNA aptamer for AMP with KD = 26 nM.•BHQ-2-(NH2)2 binding is increased in the presence of nM concentrations of AMP.•The designed aptasensor has an analytical range from 3.4 nM to 200 μM of AMP.