Knock-down of amh transcription by antisense RNA reduces FSH and increases follicular atresia in female Oreochromis niloticus

•amh gene-silencing tilapia female was constructed through antisense RNA.•amh transcription and Amh protein expression were downregulated.•AMH signaling was downregulated, and levels of LH, FSH and estradiol decreased.•Loss of Amh function caused suppression of ovarian follicular development. Anti-M...

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Bibliographic Details
Published in:Gene 2022-10, Vol.842, p.146792-146792, Article 146792
Main Authors: Qiang, Jun, Cao, Zhe-Ming, Zhu, Hao-Jun, Tao, Yi-Fan, He, Jie, Xu, Pao
Format: Article
Language:English
Subjects:
AMH signaling
amh transcription inhibition
Antisense RNA
Developmental degeneration
Oreochromis niloticus
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Summary:•amh gene-silencing tilapia female was constructed through antisense RNA.•amh transcription and Amh protein expression were downregulated.•AMH signaling was downregulated, and levels of LH, FSH and estradiol decreased.•Loss of Amh function caused suppression of ovarian follicular development. Anti-Müllerian hormone (Amh) plays an important role in regulating gonad development in teleosts. However, little is known about the effects of Amh on follicle development. In this study, we transfected the vector containing antisense RNA fragments of the amh gene to produce Nile tilapia, Oreochromis niloticus, with knocked-down Amh function in vivo. The results confirmed that the antisense RNA effectively inhibited amh transcription and Amh protein expression in female tilapia ovarian tissue. At 180 days of age, compared with control fish, female tilapia with knocked-down Amh function showed significantly increased growth and significantly decreased ovary weight and gonadosomatic index (P 
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2022.146792