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Genome-wide association study reveals that GhTRL1 and GhPIN8 affect cotton root development
Key message Two regions located at chromosome A05 and D04 were found to be significantly associated with 0–0.5 mm and 0.5–2 mm diameter roots, respectively, and two candidate genes related to root development were identified. Roots absorb water and nutrients, and play an important role in plant grow...
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Published in: | Theoretical and applied genetics 2022-09, Vol.135 (9), p.3161-3176 |
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Main Authors: | , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Key message
Two regions located at chromosome A05 and D04 were found to be significantly associated with 0–0.5 mm and 0.5–2 mm diameter roots, respectively, and two candidate genes related to root development were identified.
Roots absorb water and nutrients, and play an important role in plant growth. However, there are few genetic developmental studies on cotton root structural traits. In this study, we used 200 upland cotton (
Gossypium hirsutum
L.) varieties to analyze the phenotypic variation of 43 traits. A total of 2001 related single-nucleotide polymorphism (SNP) sites located within or near 1046 genes were detected through a genome-wide association study (GWAS). The 32 root traits were linked to SNPs that corresponded to 317 nonrepetitive genes. For SNPs associated with root length and 0–0.5 mm diameter root traits, a significant peak appeared on chromosome A05 (between 21.91 and 22.24 Mb). For SNPs associated with root surface area, root volume and 0.5–2 mm diameter root traits, a significant peak appeared on chromosome D04 (between 7.35 and 7.70 Mb). Within these two key regions, SNPs were detected in the promoter and coding regions of two candidate genes,
GhTRL1-A05
and
GhPIN8-D04
. The expression levels of these two genes also changed significantly according to transcriptome sequencing and quantitative real-time PCR (qRT–PCR). After silencing the
GhTRL1
and
GhPIN8
genes via virus-induced gene silencing (VIGS), we found that the plants expressing
TRV2::GhTRL1
and
TRV2::GhPIN8
had a reduced root length, surface area. Moreover, the contents of cis-12-oxo-phytodienoic acid (cis-OPDA), isopentenyl adenosine (iPR) and cis-zeatin (cZ) in the roots of the plants expressing
TRV2::GhTRL1
decreased. This study contributes to the cultivation and improvement of cotton varieties. |
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ISSN: | 0040-5752 1432-2242 |
DOI: | 10.1007/s00122-022-04177-x |