Bystander effect in photosensitized prostate cancer cells with a different grade of malignancy: The role of nitric oxide

• Published in: Nitric oxide 2022-11, Vol.128, p.25-36
• Main Authors: Gani, Mariachiara, Xodo, Luigi E., Rapozzi, Valentina
• Format: Article
• Language: English
• Subjects: Bystander effect; GSNOR; Nitric oxide; Photodynamic therapy; Prostate cancer cells; S-nitrosylation; Tumor malignancy
• ISSN: 1089-8603; 1089-8611
• DOI: 10.1016/j.niox.2022.08.002

Photodynamic therapy (PDT) is a therapeutic modality based on the simultaneous action of three elements: photosensitizer, light and oxygen. This triad generates singlet oxygen and reactive oxygen species that can reduce the mass of a tumor. PDT is also able to stimulate iNOS, the enzyme that generates nitric oxide (NO). The role of NO in PDT-treated cancer cells has been investigated in several studies. They showed that low iNOS/NO levels stimulate signaling pathways that promote tumor survival, while high iNOS/NO levels arrest tumor growth. There is increasing evidence that ROS/RNS control both proliferation and migration of cells in the vicinity of PDT-treated tumor cells (so-called bystander cells). In this work, we addressed the question of how NO, which is generated by weak PDT, affects bystander cells. We used a conditioned medium: medium of PDT-treated tumor cells containing the stressors produced by the cells was added to untreated cells mimicking the neighboring bystander cells to investigate whether the conditioned medium affects cell proliferation. We found that low-level NO in prostate cancer cells affects the bystander tumor cells in a manner that depends on their malignancy grade. [Display omitted] •Photodynamic therapy (PDT) and bystander effect in prostate cells.•Influence of cancer malignancy on bystander cells response to PDT.•High GSNOR levels can promote tumor progression in PC3 bystander cells.