Biofilm matrix proteome of clinical strain of P. aeruginosa isolated from bronchoalveolar lavage of patient in intensive care unit

Extracellular matrix plays a pivotal role in biofilm biology and proposed as a potential target for therapeutics development. As matrix is responsible for some extracellular functions and influence bacterial cytotoxicity against eukaryotic cells, it must have unique protein composition. P. aeruginos...

Full description

Saved in:
Bibliographic Details
Published in:Microbial pathogenesis 2022-09, Vol.170, p.105714-105714, Article 105714
Main Authors: Egorova, Daria A., Solovyev, Andrey I., Polyakov, Nikita B., Danilova, Ksenya V., Scherbakova, Anastasya A., Kravtsov, Ivan N., Dmitrieva, Maria A., Rykova, Valentina S., Tutykhina, Irina L., Romanova, Yulia M., Gintsburg, Alexander L.
Format: Article
Language:English
Subjects:
Biofilm
Cell redox homeostasis
Fatty acid synthesis
Matrix
Matrixome
Nucleoside metabolism
Proteolysis
Proteome
Pseudomonas aeruginosa
Transmembrane transport
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Extracellular matrix plays a pivotal role in biofilm biology and proposed as a potential target for therapeutics development. As matrix is responsible for some extracellular functions and influence bacterial cytotoxicity against eukaryotic cells, it must have unique protein composition. P. aeruginosa is one of the most important pathogens with emerging antibiotic resistance, but only a few studies were devoted to matrix proteomes and there are no studies describing matrix proteome for any clinical isolates except reference strains PAO1 and ATCC27853. Here we report the first biofilm matrix proteome of P. aeruginosa isolated from bronchoalveolar lavage of patient in intensive care unit. We have identified the largest number of proteins in the matrix among all published studies devoted to P. aeruginosa biofilms. Comparison of matrix proteome with proteome from embedded cells let us to identify several enriched bioprocess groups. Bioprocess groups with the largest number of overrepresented in matrix proteins were oxidation-reduction processes, proteolysis, and transmembrane transport. The top three represented in matrix bioprocesses concerning the size of the GO annotated database were cell redox homeostasis, nucleoside metabolism, and fatty acid synthesis. Finally, we discuss the obtained data in a prism of antibiofilm therapeutics development. [Display omitted]
ISSN:0882-4010
1096-1208
DOI:10.1016/j.micpath.2022.105714