Rotenoids from Clitoria fairchildiana R. Howard (Fabaceae) seeds affect the cellular metabolism of larvae of Aedes aegypti L. (Culicidae)

• Published in: Pesticide biochemistry and physiology 2022-08, Vol.186, p.105167-105167, Article 105167
• Main Authors: Bertonceli, Maria Aparecida Aride, Oliveira, Antônia Elenir Amâncio, de Souza Passos, Michel, Vieira, Ivo José Curcino, Braz-Filho, Raimundo, Lemos, Francisco José Alves, Martins, Brunna Xavier, Façanha, Arnoldo Rocha, Pireda, Saulo, da Cunha, Maura, Fernandes, Kátia Valevski Sales
• Format: Article
• Language: English
• Subjects: Butterfly pea tree; Dengue mosquito; Glucopyranosylrotenoid; Oxidative stress; V H+-ATPase activity
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2022.105167

Non-domesticated species may represent a treasure chest of defensive molecules which must be investigated and rescued. Clitoria fairchildiana R. Howard is a non-domesticated Fabacea, native from the Amazonian Forest whose seeds are exquisitely refractory to insect predation. Secondary metabolites from these seeds were fractionated by different organic solvents and the CH2Cl2 fraction (CFD – Clitoria fairchildiana dichloromethane fraction), as the most toxic to 3rd instar Aedes aegypti larvae (LC50 180 PPM), was subjected to silica gel chromatography, eluted with a gradient of CH2Cl2: MeOH and sub fractioned in nine fractions (CFD1 - CFD9). All obtained fractions were tested in their toxicity to the insect larvae. Two rotenoids, a 11α-O-β-D-glucopyranosylrotenoid and a 6-deoxyclitoriacetal 11-O-n-glucopyranoside, were identified in the mixture of CFD 7.4 and CFD 7.5, and they were toxic (LC50 120 PPM) to 3rd instar Ae. aegypti larvae, leading to exoskeleton changes, cuticular detachment and perforations in larval thorax and abdomen. These C. fairchildiana rotenoids interfered with the acidification process of cell vesicles in larvae midgut and caused inhibition of 55% of V-ATPases activity of larvae treated with 80 PPM of the compounds, when compared to control larvae. The rotenoids also led to a significant increase in the production of reactive oxygen species (ROS) in treated larvae, especially in the hindgut region of larvae intestines, indicating a triggering of an oxidative stress process to these insects. [Display omitted] •Rotenoids from Clitoria fairchildiana seeds were toxic to Aedes aegypti larvae.•Isolated rotenoids were chemically characterized as glucopyranosides.•The glucopyranosylrotenoids disrupted cell vesicles acidification in larvae midguts.•V-ATPases inhibition and ROS accumulation were caused by the rotenoids.