Quantitative proteomics reveals redox‐based functional regulation of photosynthesis under fluctuating light in plants

ABSTRACT Photosynthesis involves a series of redox reactions and is the major source of reactive oxygen species in plant cells. Fluctuating light (FL) levels, which occur commonly in natural environments, affect photosynthesis; however, little is known about the specific effects of FL on the redox r...

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Bibliographic Details
Published in:Journal of integrative plant biology 2022-11, Vol.64 (11), p.2168-2186
Main Authors: Chen, Qi, Xiao, Yixian, Ming, Yu, Peng, Rong, Hu, Jiliang, Wang, Hong‐Bin, Jin, Hong‐Lei
Format: Article
Language:English
Subjects:
Acclimation
Acclimatization
Changing environments
Environmental changes
fluctuating light
Light levels
Photosynthesis
Photosystem I
Plant cells
Proteins
Proteomes
Proteomics
Reactive oxygen species
Redox reactions
redox regulation
Switches
Switching
Thiols
Thioredoxin
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Summary:ABSTRACT Photosynthesis involves a series of redox reactions and is the major source of reactive oxygen species in plant cells. Fluctuating light (FL) levels, which occur commonly in natural environments, affect photosynthesis; however, little is known about the specific effects of FL on the redox regulation of photosynthesis. Here, we performed global quantitative mapping of the Arabidopsis thaliana cysteine thiol redox proteome under constant light and FL conditions. We identified 8857 redox‐switched thiols in 4350 proteins, and 1501 proteins that are differentially modified depending on light conditions. Notably, proteins related to photosynthesis, especially photosystem I (PSI), are operational thiol‐switching hotspots. Exposure of wild‐type A. thaliana to FL resulted in decreased PSI abundance, stability, and activity. Interestingly, in response to PSI photodamage, more of the PSI assembly factor PSA3 dynamically switches to the reduced state. Furthermore, the Cys199 and Cys200 sites in PSA3 are necessary for its full function. Moreover, thioredoxin m (Trx m) proteins play roles in redox switching of PSA3, and are required for PSI activity and photosynthesis. This study thus reveals a mechanism for redox‐based regulation of PSI under FL, and provides insight into the dynamic acclimation of photosynthesis in a changing environment. Photosystem I proteins are operational thiol‐switching hotspots under fluctuating light and the Photosystem I assembly factor PSA3 is redox‐regulated by Trx m proteins to maintain photosynthesis.
ISSN:1672-9072
1744-7909
DOI:10.1111/jipb.13348