Contextual fear conditioning regulates synapse-related gene transcription in mouse microglia

• Published in: Brain research bulletin 2022-10, Vol.189, p.57-68
• Main Authors: Yu, Zhiqian, Sakai, Mai, Fukushima, Hotaka, Ono, Chiaki, Kikuchi, Yoshie, Koyama, Ryuta, Matsui, Ko, Furuyashiki, Tomoyuki, Kida, Satoshi, Tomita, Hiroaki
• Format: Article
• Language: English
• Subjects: Fear memory; Microarray; Microglia; Synapsin; γ-aminobutyric acid (GABA)
• ISSN: 0361-9230; 1873-2747
• DOI: 10.1016/j.brainresbull.2022.08.017

Microglia have been suggested to be involved in the underlying mechanism of conditional fear memory formation by regulating inflammatory cytokines. However, the mechanism linking microglia and neuronal activity related to fear conditioning remains unclear. This study characterized the transcription profile of microglia in a fear memory conditional mouse model. Compared with those in control mice microglia, the most significantly induced genes were synapse-related, whereas immune-related genes were reduced due to fear memory consolidation. Whilst the increased expression of synapse-related genes was reversed after fear memory extinction, that of immunological genes was not, strongly suggesting a connection between microglia, neurons, and a dysregulated immune response following contextual fear conditioning. Furthermore, in the hippocampal microglia, we found that the expression of neurotransmitter release regulators, γ-aminobutyric acid (GABA) receptor GABRB3 and synapsin 1/2, increased under fear memory consolidation and restored (decreased) after extinction. In addition, compared with the transcription profile in peripheral monocytes, few overlapping genes were not enriched in biological processes. Taken together, the identified conditional fear stress-induced changes in mouse microglial transcription profiles suggest that microglia-neuron communication mediates contextual fear conditioning. •Synapse-related genes in microglia are changed by contextual fear conditioning.•Consolidation of fear memory induces immune dysfunction in microglia.•Microglial GABRB3 and Synapsin are changed during contextual fear conditioning.•Transcriptional profiles in microglia are different from the peripheral monocytes.