Generation of 3′-OH terminal–triggered encoding of multicolor fluorescence for simultaneous detection of different DNA glycosylases

• Published in: Analytical and bioanalytical chemistry 2022-09, Vol.414 (23), p.6989-7000
• Main Authors: Zhang, Huige, Gao, Zixi, He, Fei, Lan, Jingfeng, Chai, Hailong, Zhang, Shiqian, Zuo, Xianwei, Chen, Hongli, Chen, Xingguo
• Format: Article
• Language: English
• Subjects: Analysis; Analytical Chemistry; Biochemistry; Cancer; Cervical cancer; Cervical carcinoma; Cervix; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Deoxyribonucleic acid; Detection limits; DNA; DNA damage; DNA glycosylase; DNA glycosylases; DNA repair; Enzyme kinetics; Epithelial cells; Epithelium; Fluorescence; Food Science; Gene expression; Immunodeficiency; Laboratory Medicine; Lymphocytes; Lymphocytes T; Magnetic separation; Methods; Monitoring/Environmental Analysis; Movement disorders; Neurodegenerative diseases; Parkinson's disease; Research Paper; Uracil; Uracil-DNA glycosidase
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-022-04267-1

Uracil DNA glycosylase (UDG) and human alkyladenine DNA glycosylase (hAAG) are the important DNA glycosylases for initiating the repair of DNA damage, and the aberrant expression of DNA glycosylases is closely associated with various diseases, such as Parkinson’s disease, several cancers, and human immunodeficiency. The simultaneous detection of UDG and hAAG is helpful for the study of early clinical diagnosis. However, the reported methods for multiple DNA glycosylase assay suffer from the application of an expensive single-molecule instrument, labor-tedious magnetic separation, and complicated design. Herein, we develop a simple fluorescence method with only three necessary DNA strands for the selective and sensitive detection of multiple DNA glycosylase activity based on the generation of 3′-OH terminal–triggered encoding of multicolor fluorescence. The method can achieve the detection limits of 5.5 × 10 −5 U/mL for UDG and 3.3 × 10 −3 U/mL for hAAG, which are lower than those of the reported fluorescence methods. Moreover, it can be further used to detect multiple DNA glycosylases in the human cervical carcinoma cell line (HeLa cells), normal human renal epithelial cells (293 T cells), and biological fluid and measure the enzyme kinetic parameters of UDG and hAAG. Graphical abstract