Cyanidin-3-O-glucoside promotes stress tolerance and lifespan extension of Caenorhabditis elegans exposed to polystyrene via DAF-16 pathway

• Published in: Mechanisms of ageing and development 2022-10, Vol.207, p.111723-111723, Article 111723
• Main Authors: Chen, Wen, Chen, Zhen, Shan, Shuo, Wu, Aibo, Zhao, Chao, Ye, Xiang, Zheng, Xiaodong, Zhu, Ruiyu
• Format: Article
• Language: English
• Subjects: Cyanidin-3-O-glucoside; DAF-16 pathway; Lifespan extension; Polystyrene; Stress resistance
• ISSN: 0047-6374; 1872-6216
• DOI: 10.1016/j.mad.2022.111723

Microplastic pollution has attracted growing attention due to its prevalent and persistent exposure to general population through the food chain, but few reports have focused on the toxicological prevention of polystyrene (PS). Using the wild-type and mutant strains, this study explored the impacts of PS and cyanidin-3-O-glucoside (C3G) on stress tolerance and lifespan of Caenorhabditis elegans (C. elegans). In N2 nematodes, PS exposure initiated the oxidative stress and subsequent lifespan reduction, while these adverse impacts could be positively improved by C3G treatment. Considering the pivotal role of DAF-16 pathway in stress tolerance and lifespan regulation, the expression of the daf-16 gene and its downstream antioxidant genes (clt-2, hsp-16.1, sod-3, sod-5) were examined, and found to be significantly enhanced by C3G. Since the sod-3 gene was up-regulated the most fold by C3G, the activity of SOD enzyme that encoded by the sod-3 was examined, and could be obviously enhanced upon C3G treatment. This explained the improved oxidative stress and delayed oxidation-associated aging after C3G intervention. Nevertheless, these positive effects of C3G were weakened in daf-16(-) mutant strain (with deleted DAF-16 gene), for which the beneficial effects of C3G in promoting stress resistance and lifespan extension were inhibited. These findings suggested that the DAF-16 gene and its downstream antioxidant genes, have participated in C3G’s regulations on redox balance and lifespan that impacted by nano-polystyrene particles. This study highlighted the link between dietary components and environmentally driven disturbance. [Display omitted] •PS induced oxidative stress and shortened lifespan of nematodes were reversed by C3G.•C3G-mediated lifespan extension involves DAF-16 and its downstream antioxidant genes.•Involvements of DAF-16 and downstream antioxidant gene were verified in daf-16(-) mutant.•The links between nanoparticles and dietary anthocyanin are highlighted in this work.