Binding of rosmarinic acid curcumin and capsaicin with PLA2: A comparative study

Phospholipase A2 (PLA2) is a key enzyme involved in the formation of pro-inflammatory mediators like eicosanoids. Inhibition of PLA2 is regarded as one of the effective methods of controlling inflammation. The present study investigated the binding potentials of three natural compounds, rosmarinic a...

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Published in:Biochemical and biophysical research communications 2022-10, Vol.626, p.187-191
Main Authors: Aiswarya, N., Remya, Chandran, Remashree, A.B., Sadasivan, C., Dileep, K.V.
Format: Article
Language:English
Subjects:
Capsaicin
Capsaicin and molecular docking
Cinnamates
Curcumin
Curcumin - chemistry
Curcumin - pharmacology
Depsides
Phospholipase A2
Phospholipases A2 - metabolism
Rosmarinic Acid
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Summary:Phospholipase A2 (PLA2) is a key enzyme involved in the formation of pro-inflammatory mediators like eicosanoids. Inhibition of PLA2 is regarded as one of the effective methods of controlling inflammation. The present study investigated the binding potentials of three natural compounds, rosmarinic acid (RA), capsaicin (CAP), and curcumin (CUR) by means of in silico and in vitro methods. Our study revealed that RA has relatively better binding affinity and inhibition potentials when compared to the other two molecules. Our ITC experiments were also suggested a slightly better binding energy for the RA. The stoichiometry of the protein ligand complex obtained from one of the ITC experiments suggested the possibilities of binding of a small molecule MCW (degraded product of CUR) on PLA2. Overall study demonstrated that the anti-inflammatory activity of RA, CUR and CAP may be partly due to the inhibition of PLA2. [Display omitted] •Anti-inflammatory activity of Rosmarinic acid, Curcumin and Capsaicin may be partly due to the inhibition of sPLA2.•Rosmarinic acid possess has slightly better binding affinity than the other selected compounds.•These molecules could be used as health supplement that act as protective agent/immune booster/anti-inflammatory agents.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2022.08.028