Ethanol Extract of Glycyrrhiza uralensis Fisch: Antidiarrheal Activity in Mice and Contraction Effect in Isolated Rabbit Jejunum

Objective To evaluate the antidiarrheal effect of ethanol extract of Glycyrrhiza uralensis Fisch root (GFR) in vivo and jejunal contraction in vitro . Methods In vivo , 50 mice were divided into negative control, positive control (verapamil), low-, medium- and high-dose GFR (250, 500, 1,000 mg/kg) g...

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Bibliographic Details
Published in:Chinese journal of integrative medicine 2023-04, Vol.29 (4), p.325-332
Main Authors: Wen, Jing, Zhang, Jian-wu, Lyu, Yuan-xia, Zhang, Hui, Deng, Kai-xi, Chen, Hong-xue, Wei, Ying
Format: Article
Language:English
Subjects:
Animals
Antidiarrheals - adverse effects
Calcium Chloride - adverse effects
Castor Oil - adverse effects
Diarrhea - drug therapy
Glycyrrhiza uralensis
Jejunum
Medicine
Medicine & Public Health
Mice
Muscle Contraction
Original Article
Plant Extracts - adverse effects
Rabbits
Verapamil - adverse effects
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Summary:Objective To evaluate the antidiarrheal effect of ethanol extract of Glycyrrhiza uralensis Fisch root (GFR) in vivo and jejunal contraction in vitro . Methods In vivo , 50 mice were divided into negative control, positive control (verapamil), low-, medium- and high-dose GFR (250, 500, 1,000 mg/kg) groups by a random number table, 10 mice in each group. The antidiarrheal activity was evaluated in castor oil-induced diarrhea mice model by evacuation index (EI). In vitro , the effects of GFR (0.01, 0.03, 0.1, 0.3, 1, 3, and 10 g/L) on the spontaneous contraction of isolated smooth muscle of rabbit jejunum and contraction of pretreated by Acetylcholine (ACh, 10 µmol/L) and KCl (60 mmol/L) were observed for 200 s. In addition, CaCl 2 was accumulated to further study its mechanism after pretreating jejunal smooth muscle with GFR (1 and 3 g/L) or verapamil (0.03 and 0.1 µmol/L) in a Ca 2+ -free-high-K + solution containing ethylene diamine tetraacetic acid (EDTA). Results GFR (500 and 1,000 mg/kg) significantly reduced EI in castor oil-induced diarrhea model mice ( P
ISSN:1672-0415
1993-0402
DOI:10.1007/s11655-022-3536-5