Characterization of a new NSP2-deletion NADC34-Like Porcine Reproductive and Respiratory Syndrome Virus in China

• Published in: Research in veterinary science 2022-12, Vol.152, p.212-218
• Main Authors: Xie, Chang-Zhan, Tao, Yi-Mo, Ha, Zhuo, Zhang, Ping, Zhang, Ying, Zhang, He, Jin, Ning-Yi, Lu, Hui-Jun
• Format: Article
• Language: English
• Subjects: Amino acids; Animal diseases; Deletion; Economic impact; Epitopes; Evolution & development; Genes; Genomes; Glycosylation; Hogs; Homology; Insertion; Morbidity; Mutation; NADC34-Like strain; Neutralizing; Next-generation sequencing; NSP2-deletion; Nucleotide sequence; Nucleotides; Pathogenicity; Pathogens; Peptides; Phylogenetics; Proteins; PRRSV; Recombination; Respiratory diseases; Strains (organisms); Veterinary medicine; Viruses
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2022.08.001

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is the causative agent of Porcine Reproductive and Respiratory Syndrome (PRRS), which has caused huge economic losses to the pig industry worldwide. PRRSV NADC34-Like PRRSV 2020-Acheng-1 strain, which caused high morbidity and high mortality were isolated from dead piglets (high-throughput sequencing to show that only PRRSV and TGEV) on a farm in northeastern China. The full-length genome sequence of 2020-Acheng-1 shares 95.6% nucleotide homology with NADC34 PRRSV without any gene insertion, but has a unique 17 amino acid (469aa to 486aa) deletion in Nsp2 compared with all NADC34-Like strains in NCBI and there are unique 100 amino acid deletions. In addition, difference degree of changes in signal peptide, trans-membrane region (TM), main neutralizing epitope (PNE), non-neutralizing epitope and N-glycosylation site were observed in GP5 of 2020-Acheng-1 and other PRRSV-2 strains, we only found a change in the fifteenth amino acid of signal peptide of in GP5 of 2020-Acheng-1 with NADC34 strains. Recombination analysis showed that 2020-Acheng-1 strain did not have any recombination events with representative PRRSV-2 strains in China. This study provided valuable evidence for understanding the role of NADC34-Like strain that impact on pathogenicity. •The first time we found NSP2 of 2020-Acheng-1 strain appeared 17 amino acids deletion at 469–486 with other NADC34-Like strain.•The first time that very serious lung pathological changes caused by the NADC34-Like strain were found in China.•It is proved that the NADC34-Like strain has been trending in Heilongjiang Province, China.