In vivo efficacy of verteporfin loaded gold nanorods for combined photothermal/photodynamic colon cancer therapy

• Published in: International journal of pharmaceutics 2022-09, Vol.625, p.122134-122134, Article 122134
• Main Authors: Licciardi, Mariano, Varvarà, Paola, Tranchina, Luigi, Puleio, Roberto, Cicero, Luca, Cassata, Giovanni, Giammona, Gaetano
• Format: Article
• Language: English
• Subjects: Gellan gum; Gold nanorods; Human colon cancer; Photodynamic therapy; Photothermal therapy; Verteporfin
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2022.122134

[Display omitted] •Combined photothermal/photodynamic therapy could improve cancer treatment efficacy.•Natural coatings allow gold nanorods (AuNRs) in vivo administration.•Verteporfin is an intriguing agent for the photodynamic therapy of colon cancer.•Drug-free AuNRs decelerate colon cancer growth via photothermal therapy.•Verteporfin-AuNRs eradicate colon cancer through photothermal/photodynamic therapy. The high incidence of cancer recurrences and the frequent occurrence of multidrug resistance often stem from a poorly selective and inefficient antineoplastic therapy, responsible for the onset of undesired side effects as well. A combination of minimal-invasive approaches could thus be a useful strategy to surmount these shortcomings, achieving a safe and solid cancer therapy. Herein, a multi-therapeutic nanotool was designed by merging the photothermal properties of gold nanorods (AuNRs) with the photodynamic activity of the photosensitizer verteporfin. AuNRs were coated with the natural materials lipoic acid and gellan gum (AuNRs_LA,GG) and subsequently loaded with verteporfin (AuNRs_LA,GG/Vert) producing stable colloidal dispersions. AuNRs_LA,GG/Vert were characterized in terms of stability, size and morphology. The hyperthermia exhibited after NIR excitation (810 nm) was also evaluated to highlight the effect on increasing the drug released profile in intra-tumoral mimicking media, as well as cytotoxicity on human colon cancer cell line (HCT116). In vivo studies on HCT116 murine xenograft models were carried out to prove the ability of AuNRs_LA,GG to arrest the tumor growth via NIR laser-triggered hyperthermia. Furthermore, the complete xenograft depletion was demonstrated upon AuNRs_LA,GG/Vert administration by combined photothermal (PTT) and photodynamic (PDT) effects.